Comprehensive analysis and expression profile of the homeodomain leucine zipper IV transcription factor family in tomato
Comprehensive analysis and expression profile of the homeodomain leucine zipper IV transcription factor family in tomato
复制标题
番茄同源域亮氨酸拉链IV转录因子家族的综合分析和表达谱
DOI：
10.1016/j.plaphy.2015.07.025
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发表时间：
2015-11-01
影响因子：
6.5
通讯作者：
Yang, Changxian
中科院分区：
文献类型：
--
作者：
Gao, Yanna;Gao, Shenghua;Yang, Changxian
同源结构域亮氨酸拉链 IV (HD-ZIP IV) 蛋白是植物特异性转录因子，在表皮细胞层发育和角质层形成中发挥重要作用。番茄 (Solanum lycopersicum) 中两个 HD-ZIP IV 家族基因 CD2 和 Wo 的功能已得到充分表征。 CD2 和 Wo 分别参与角质层生物合成和毛状体形成。在这项研究中，我们鉴定了 13 个新的番茄 HD-ZIP IV (SlHDZIV) 基因。我们分析了这些 SlHDZIV 基因的结构、染色体位置、系统发育、蛋白质基序和表达谱。基因结构分析表明，SlHDZIV基因存在11个外显子和10个内含子的模块。这些基因不对称地分布在除 4 号和 5 号染色体外的染色体上。节段重复可能导致番茄 HD-ZIP IV 基因的扩展。这些基因的表达谱揭示了它们广泛的表达模式以及在幼叶和花中的高表达。每个基因对一种以上不同的植物激素[脱落酸、乙烯利、4-(吲哚基)-丁酸、茉莉酸、水杨酸、赤霉酸和6-苄氨基嘌呤]和四种非生物胁迫处理(冷、热、盐和干旱)作出反应。这项研究为 SlHDZIV 基因在番茄生长和发育中的不同作用提供了重要的见解。 (C) 2015 Elsevier Masson SAS。版权所有。
Homeodomain leucine zipper IV (HD-ZIP IV) proteins are plant-specific transcription factors that play important roles in development of epidermal cell layers and cuticle formation. The functions of two HD-ZIP IV family genes, CD2 and Wo, have been well characterized in tomato (Solanum lycopersicum). CD2 and Wo are involved in cuticle biosynthesis and trichome formation, respectively. In this study, we identified 13 novel tomato HD-ZIP IV (SlHDZIV) genes. We analyzed the structures, chromosome locations, phylogeny, protein motifs, and expression profiles of these SlHDZIV genes. Gene structure analysis revealed that a module of 11 exons and 10 introns existed in the SlHDZIV genes. These genes were asymmetrically distributed on chromosomes, except on chromosome 4 and 5. Segmental duplication possibly contributed to the expansion of tomato HD-ZIP IV genes. The expression profiles of these genes revealed their broad expression pattern and high expression in young leaves and flowers. Each gene responded to more than one of different phytohormones [abscisic acid, ethephon, 4-(indolyl)-butyric acid, jasmonic acid, salicylic acid, gibberellic acid, and 6-benzylaminopurine] and four abiotic stress treatments (cold, heat, salt, and drought). This study provided significant insights into the diverse roles of SlHDZIV genes in tomato growth and development. (C) 2015 Elsevier Masson SAS. All rights reserved.