Establishment and cryopreservation of liver, heart and muscle cell lines derived from the Chinese alligator (Alligator sinensis)
Establishment and cryopreservation of liver, heart and muscle cell lines derived from the Chinese alligator (Alligator sinensis)
复制标题
扬子鳄肝脏、心脏和肌肉细胞系的建立和冷冻保存
DOI：
10.1007/s11434-011-4622-9
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发表时间：
2011-08
影响因子：
--
通讯作者：
Fang ShengGuo
中科院分区：
文献类型：
--
作者：
Zeng ChangJun;Ye Qing;Fang ShengGuo
扬子鳄（Alligator sinensis）是一种极度濒危物种。濒危物种基因资源保护工程首先涉及器官、组织、配子、基因组DNA文库和细胞系的保护。本研究首次建立了扬子鳄肝脏、心脏和肌肉组织细胞系并进行了冻存。研究表明，肝（11-12 d）、心脏（13-14 d）和肌肉（17-18 d）组织块在原代培养中的细胞迁移时间存在很大差异。原代细胞培养时间的差异表明，建立爬行动物内脏来源的细胞系相对容易。生物学分析表明，解冻细胞的群体倍增时间约为36 h。染色体核型分析表明，扬子鳄细胞染色体数目为2n=32的频率为88.6%~ 93.4%。本研究建立的扬子鳄细胞系为研究扬子鳄提供了重要的细胞资源，对保护这一珍稀濒危物种具有重要意义。这些方法的建立为爬行动物遗传资源的细胞水平保存提供了技术和理论支持。
The Chinese alligator,Alligator sinensis, is a critically endangered species. A conservation project of gene resources for an endangered species first involves the preservation of organs, tissues, gametes, genomic DNA libraries and cell lines. The present study is the first to establish and cryopreserve cell lines of liver, heart and muscle tissues from the Chinese alligator. The study revealed that there was a large discrepancy in cell migration time in primary cultures among liver (11–12 d), heart (13–14 d) and muscle (17–18 d) tissue pieces. The differences in time in primary cell culture suggested that it was relatively easy to build visceral-derived cell lines for reptiles. Biological analysis showed that the population doubling time for thawed cells was approximately 36 h. Karyotyping revealed that the frequency of Chinese alligator cells showing chromosome number as 2n=32 was 88.6%–93.4%. Chinese alligator cell lines established here provide a vital resource for research and are likely to be useful for protection of this rare and critically endangered species. Furthermore, the establishment of these methods may supply technical and theoretical support for preserving genetic resources at the cellular level for other reptile species.
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影响因子：
5.9
作者：
J. Thorbjarnarson;Xiaoming Wang;S. Ming;Lijun He;Youzhong Ding;Yue-Kun Wu;S. Mcmurry
通讯作者：
J. Thorbjarnarson;Xiaoming Wang;S. Ming;Lijun He;Youzhong Ding;Yue-Kun Wu;S. Mcmurry
影响因子：
8.5
作者：
Guan, W.;He, X.;Ma, Y. -H.
通讯作者：
Ma, Y. -H.
DOI：
10.1152/ajplegacy.1960.198.4.861
发表时间：
1960-04
期刊：
The American journal of physiology
影响因子：
--
作者：
C. Wilber
通讯作者：
C. Wilber
影响因子：
1.5
作者：
M. King;R. Honeycutt;N. Contreras
通讯作者：
M. King;R. Honeycutt;N. Contreras
DOI：
--
发表时间：
2004
期刊：
Chinese Biodiversity
影响因子：
--
作者：
D. You
通讯作者：
D. You