Enzymatic Synthesis of Two Ginsenoside Re-β-xylosides
Enzymatic Synthesis of Two Ginsenoside Re-β-xylosides
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DOI：
10.1271/bbb.65.1223
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发表时间：
2001-01
期刊：
影响因子：
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通讯作者：
S. Ko;Yukio Suzuki;Young-Hoi Kim;Kang-Ju Choi
中科院分区：
文献类型：
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作者：
S. Ko;Yukio Suzuki;Young-Hoi Kim;Kang-Ju Choi
20（S）-原人参三醇6-O-α-L-吡喃鼠李糖基-（1→2）-[β-D-吡喃木糖基-（1→4）]-β-D-吡喃葡萄糖基-20-O-β-D-吡喃葡萄糖苷和20（S）-原人参三醇6-O-α-L-吡喃鼠李糖基-（1→2）-[β-D-吡喃木糖基-（1→6）]-β-D-吡喃葡萄糖基-20-O-β-D-吡喃葡萄糖苷，分别以对硝基苯基β-D-吡喃木糖苷和苯基β-D-吡喃木糖苷为供体，β-Re为受体，在25%丙酮和乙腈中，用绿色木霉纤维素酶和黑曲霉β-半乳糖苷酶合成了β-D-吡喃木糖苷。后者的酶制剂也催化水解的皂苷Re的次要皂苷，皂苷Rg 2。
Two new β-xylosyl derivatives of ginsenoside Re, 20(S)-protopanaxatriol 6-O-α-L-rhamnopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→4)]-β-D-glucopyranosyl-20-O-β-D-glucopyranoside and 20(S)-protopanaxatriol 6-O-α-L-rhamnopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→6)]-β-D-glucopyranosyl-20-O-β-D-glucopyranoside, were respectively synthesized from p-nitrophenyl β-D-xylopyranoside and phenyl β-D-xylopyranoside as donors and ginsenoside Re as the acceptor in 25% acetone and acetonitrile by a cellulase preparation from Trichoderma viride and a β-galactosidase preparation from Aspergillus oryzae. The latter enzyme preparation also catalyzed the hydrolysis of ginsenoside Re to the minor saponin, ginsenoside Rg2.