Crocetin protects against mitochondrial damage induced by UV-A irradiation in corneal epithelial cell line HCE-T cells
Crocetin protects against mitochondrial damage induced by UV-A irradiation in corneal epithelial cell line HCE-T cells
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DOI：
10.1016/j.jphs.2022.10.005
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发表时间：
2022-10-28
影响因子：
3.5
通讯作者：
Hara, Hideaki
中科院分区：
文献类型：
--
作者：
Otsu, Wataru;Yako, Tomohiro;Hara, Hideaki
角膜上皮位于眼表的最外层，并不断暴露于环境因素，如来自阳光的紫外线（UV）辐射。紫外线照射导致细胞内活性氧（ROS）的过量产生，从而导致线粒体等膜结合细胞器的氧化损伤，最终导致细胞死亡。Crocetin是一种在植物中发现的天然类胡萝卜素，具有多种生物特性，包括抗氧化活性。在这项研究中，我们研究了紫荆素对uv - a诱导的角膜上皮细胞损伤的影响。在人角膜上皮细胞转化（HCE-T）细胞系的体外系统中，10 mM crocetin预处理可抑制UV-A暴露诱导的细胞活力降低。Crocetin改善了在UV-A照射后发生的氧气消耗率和线粒体断裂的降低。Crocetin抑制ROS的产生和细胞凋亡通路的激活；它还保留了uv - a照射后HCE-T细胞上皮细胞极性和屏障功能的缺陷。在西红花素的作用下，根尖Mucin-16表达的减少部分恢复。综上所述，我们认为crocetin对UV-A照射诱导的角膜上皮细胞线粒体损伤具有保护作用。(c) 2022作者。由Elsevier B.V.代表日本药理学会制作和主办。这是一篇基于CC BY-NC-ND许可的开放获取文章（http://creativecommons.org/lice nses/ BY-NC-ND /4.0/）。
The corneal epithelium is located at the outermost layer of the ocular surface and continuously exposed to environmental factors, such as ultraviolet (UV) radiation from sunlight. UV irradiation causes excessive production of reactive oxygen species (ROS) in cells, which results in oxidative damage to membrane -bound organelles such as mitochondria, eventually leading to cell death. Crocetin, a natural carotenoid found in plants, has various biological properties including antioxidant activity. In this study, we investigated the effects of crocetin on UV-A-induced cell injury in the corneal epithelium. Using an in vitro system with the human corneal epithelial cell-transformed (HCE-T) cell line, pretreatment with 10 mM crocetin suppressed the reduction of cell viability induced by UV-A exposure. Crocetin ameliorated the decrease in oxygen consumption rates and the mitochondrial fragmentation that occurred following UV-A irradiation. Crocetin inhibited both ROS production and the activation of the apoptosis pathway; it also preserved the defects of epithelial cell polarity and barrier function in UV-A-irradiated HCE-T cells. The reduction in apical Mucin-16 expression was partially recovered in the presence of crocetin. Taking these findings together, we conclude that crocetin has a protective effect against UV-A irradiation -induced mitochondrial injury in corneal epithelial cells.(c) 2022 The Authors. Production and hosting by Elsevier B.V. on behalf of Japanese Pharmacological Society. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/lice nses/by-nc-nd/4.0/).