Adam17, a Target of Mir-326, Promotes Emt-Induced Cells Invasion in Lung Adenocarcinoma
Adam17, a Target of Mir-326, Promotes Emt-Induced Cells Invasion in Lung Adenocarcinoma
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DOI：
10.1159/000430288
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发表时间：
2015-01-01
影响因子：
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通讯作者：
Weng, Yuan
中科院分区：
文献类型：
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作者：
Cai, Ming;Wang, Zhiqiang;Weng, Yuan
背景/目的：一种崩解素和金属蛋白酶（adam17）已被报道与癌细胞侵袭有关。然而，其在肺腺癌中的潜在作用尚未明确。方法：采用RT-PCR和Western blot检测73例肺腺癌标本中miR-326和ADAM17的表达。miR-326模拟物和抑制物转染人A549和SPCA1细胞系。transwell法检测细胞侵袭能力。采用荧光素酶报告基因法评价其调控机制。采用Western blot法检测上皮-间质转化（EMT）标志物。结果：我们发现ADAM17在肺腺癌和细胞系中表达增加。在体外，上调ADAM17促进细胞侵袭，而沉默ADAM17则抑制细胞侵袭。同时，ADAM17可以影响EMT的标志物。此外，我们证实ADAM17是miR-326的靶标，miR-326参与EMT和细胞侵袭。结论：这些发现表明，miR-326的靶点ADAM17促进了emt诱导的肺腺癌细胞的侵袭。巴塞尔S. Karger股份有限公司版权所有
Background/Aims: A disintegrin and metalloprotease (ADAM) 17 has been reported to be implicated in cancer cells invasion. Nevertheless, its potential role in lung adenocarcinoma has not been addressed clearly. Methods: RT-PCR and Western blot were used to detect the expression of miR-326 and ADAM17 in lung adenocarcinoma samples (n=73). miR-326 mimics and inhibitor were tansfected in human A549 and SPCA1 cell lines. The transwell assay was used to detect the cell invasive ability. The regulation mechanism was evaluated by luciferase reporter assay. The markers of (epithelial-to-mesenchymal transition) EMT were detected by using Western blot assay. Results: We found increased expression of ADAM17 in lung adenocarcinoma and cell lines. In vitro, up-regulation of ADAM17 promoted cells invasion, while silencing of ADAM17 inhibited cells invasion. Meanwhile, ADAM17 could affect the markers of EMT. Furthermore, we confirmed that ADAM17 is a target of miR-326, which is involved in EMT and cells invasion. Conclusions: These findings revealed that ADAM17, a target of miR-326, promoted EMT-induced cells invasion in lung adenocarcinoma. Copyright (C) 2015 S. Karger AG, Basel