In Vitro antibacterial effects of antilipopolysaccharide DNA aptamer-C1qrs complexes
In Vitro antibacterial effects of antilipopolysaccharide DNA aptamer-C1qrs complexes
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DOI：
10.1007/s12223-008-0046-6
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发表时间：
2008-07-01
影响因子：
2.6
通讯作者：
Phillips, T.
中科院分区：
文献类型：
--
作者：
Bruno, J. G.;Carrillo, M. P.;Phillips, T.
DNA 适体是针对大肠杆菌 O111:B4 的脂多糖 (LPS) 开发的，并通过基于比色酶的微孔板测定显示可以结合 LPS 和大肠杆菌。多克隆适体直接使用双功能接头或间接使用生物素化适体和链霉亲和素-C1qrs 复合物与人 C1qrs 蛋白偶联。当在人血清存在下对大肠杆菌 O111:B4 和 K12 菌株进行一系列 10 倍稀释时，这两个系统均显着减少了菌落计数；将其按 1:10(3) 稀释，以避免补体激活的竞争性替代途径导致显着的细菌裂解。从抗 LPS 适体库中克隆并测序了许多候选 DNA 适体序列，用于未来筛选抗菌或“抗生素”潜力，并帮助最终开发抗生素耐药性细菌感染的替代疗法。
DNA aptamers were developed against lipopolysaccharide (LPS) from E. coli O111:B4 and shown to bind both LPS and E. coli by a colorimetric enzyme-based microplate assay. The polyclonal aptamers were coupled to human C1qrs protein either directly using a bifunctional linker or indirectly using biotinylated aptamers and a streptavidin-C1qrs complex. Both systems significantly reduced colony counts when applied to E. coli O111:B4 and K12 strains across a series of 10x dilutions of the bacteria in the presence of human serum; it was diluted 1: 10(3) in order to avoid significant bacterial lysis by the competing alternate pathway of complement activation. A number of candidate DNA aptamer sequences were cloned and sequenced from the anti-LPS aptamer library for future screening of antibacterial or "antibiotic" potential and to aid in eventual development of an alternative therapy for antibiotic-resistant bacterial infections.