A novel metagenome-derived gene cluster from termite hindgut: Encoding phosphotransferase system components and high glucose tolerant glucosidase
A novel metagenome-derived gene cluster from termite hindgut: Encoding phosphotransferase system components and high glucose tolerant glucosidase
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DOI：
10.1016/j.enzmictec.2015.12.005
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发表时间：
2016-03-01
影响因子：
3.4
通讯作者：
Li, Gang
中科院分区：
文献类型：
--
作者：
Gao, Gang;Wang, An;Li, Gang
对白蚁后肠宏基因组文库的功能筛选发现了编码磷酸转移酶系统（PTS）成分的重叠基因簇，该基因簇由糖苷特异性PTS酶II基因（glu1923）和糖苷水解酶基因（glu1392）组成。水解实验表明，Glul 923 和 Glu1392 的联合作用是重组大肠杆菌 (E. coli) 菌株中糖苷底物利用的原因。酵母二杂交分析表明Glu1923和Glu1392之间存在相互作用，并且Glu1923的EIIA结构域在相互作用中发挥了重要作用。此外，水解酶Glu1392对纤维二糖和麦芽糖表现出水解能力，并且对葡萄糖具有非常高的耐受性，K-i值为2.25 M。经过进一步研究，这些特性使Glu1392成为生物技术应用中有趣的候选者。 (C) 2015 Elsevier Inc. 保留所有权利。
Functional screening of a metagenomic library of termite hindgut identified an overlapping gene cluster encoding the phosphotransferase system (PTS) components, which consisted of a glucoside specific PTS enzyme II gene (glu1923) and a glycoside hydrolase gene (glu1392). Hydrolytic experiments revealed that the combined effect of Glul 923 and Glu1392 was responsible for the utilization of glucosidic substrates in recombinant Escherichia coli (E. coli) strains. Yeast two hybrid analysis suggested that there was an interaction between Glu1923 and Glu1392, and the domain EIIA of Glu1923 played an important role for the interaction. In addition, the hydrolase Glu1392 displayed hydrolysis ability toward cellobiose and maltose, and had a very high tolerance to glucose with a K-i value of 2.25 M. These properties make Glu1392 an interesting candidate in biotechnology applications after further study. (C) 2015 Elsevier Inc. All rights reserved.