Formation of an Antral Follicle-Like Structure by Bovine Cumulus-Oocyte Complexes Embedded with Fragmin/Protamine Microparticles.
Formation of an Antral Follicle-Like Structure by Bovine Cumulus-Oocyte Complexes Embedded with Fragmin/Protamine Microparticles.
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嵌入法安明/鱼精蛋白微粒的牛卵丘-卵母细胞复合物形成窦卵泡样结构。
DOI：
10.1080/10495398.2015.1015681
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Otoi T
中科院分区：
文献类型：
--
作者：
Morita Y;Karja NW;Do LT;Tanihara F;Taniguchi M;Otoi T
通过将法安明与鱼精蛋白简单混合，制备直径约为0.5-1 µM的法安明/鱼精蛋白微粒（F/P MP）。本研究探讨了含F/P MP的胶原凝胶作为激素载体对牛卵母细胞窦状卵泡样结构形成和生长发育的影响。在胶原凝胶中添加F/P MPs有助于卵泡刺激素（FSH）对窦状卵泡样结构的形成和大小的有益作用。F/PMPs可作为牛早期有腔卵泡卵母细胞体外培养的激素载体。
Fragmin/protamine microparticles (F/P MPs) approximately 0.5-1 µM in diameter were prepared by the simple mixing of fragmin with protamine. This study investigated the effects of F/P MP-containing collagen gels as a hormone carrier on the formation of antral follicle-like structures and on the development of growing bovine oocytes. The supplementation of F/P MPs in collagen gels contributed to the beneficial effects offollicle stimulating hormone (FSH) on the formation and size of antral follicle-like structures. The F/P MPs may serve as potential hormone carriers for the growth of cultured bovine oocytes from early antral follicles.