The Effect of Sevoflurane Inhalation on GABAergic Neurons Activation: Observation on the GAD67-GFP Knock-In Mouse
The Effect of Sevoflurane Inhalation on GABAergic Neurons Activation: Observation on the GAD67-GFP Knock-In Mouse
复制标题
吸入七氟醚对 GABA 能神经元激活的影响：GAD67-GFP 敲入小鼠的观察
DOI：
10.1002/ar.21113
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发表时间：
2010-12-01
影响因子：
2
通讯作者：
Wu, Sheng-Xi
中科院分区：
文献类型：
--
作者：
Han, Li-Chun;Zhang, Hui;Wu, Sheng-Xi
挥发性麻醉剂的潜在机制尚未很好地阐明。新出现的研究主要集中在γ-氨基丁酸(GABA)神经元的参与，但尚缺乏形态学证据。为从形态上阐明七氟醚吸入对GABA能神经元的激活作用，观察了七氟醚吸入后谷氨酸脱羧酶(GAD)67-GFP基因敲除小鼠脑内Fos和GABA能神经元的双重标记。将20只GAD67-GFP基因敲除小鼠分为三组：SI组：七氟醚诱导的不完全麻醉状态；S2组：七氟醚诱导的完全麻醉状态；与C组比较，七氟醚组大鼠下丘脑背内侧核(DM)、中脑导水管周围灰质(PAG)、海马区(CA1、DG)、室旁核(PV)、外侧隔核(LS)、扣带回(CG1、CG2)Fos表达显著增加，S2组Fos表达增强。在S2组，Fos仅表达于杏仁内侧核(Mea)、Edinger-Westphal核(E-W)、弓状核(Arc)和下丘脑室旁核(PAV)腹侧部。免疫荧光双重染色显示，在LS中，几乎所有的Fos都存在于GABA能神经元中。在CA1、DG、DM、CGL、CG2和PAG也有Fos表达，但在GABA能神经元中仅有少量表达。FOS在丘脑有很高的表达，但未发现共存，在该区域未检测到GABA能神经元。我们的结果为特定脑区的GABA能传递可能参与七氟醚麻醉提供了形态学证据。Anat Rec，293：2114-2122,2010。(C)2010年Wiley-Liss公司
The mechanisms underlying volatile anesthesia agents are not well elucidated. Emerging researches have focused on the participation of gamma-aminobutyric acid (GABA) neurons but there still lacks morphological evidence. To elucidate the possible activation of GABAergic neurons by sevoflurane inhalation in morphology, Fos (as neuronal activity marker) and GABA neurons double labeling were observed on the brain of glutamic acid decarboxylase (GAD) 67-GFP knock-in mice after sevoflurane inhalation. Twenty GAD67-GFP knock-in mice were divided into three groups: Si group: incomplete anesthesia state induced by sevoflurane; S2 group: complete anesthesia state induced by sevoflurane; control(C) group. Sevoflurane induced a significant increase of Fos expression in the dorsomedial hypothalamic nucleus (DM), periaqueductal grey (PAG), hippocampus (CA1, DG), paraventricular thalamic nucleus (PV), lateral septal nucleus (LS), and cingulate cortex (Cgl and Cg2) in Si group compared to C group, and increase of Fos expression in S2 group compared to Si group. In S2 group, Fos was only expressed in the medial amygdaloid nucleus (MeA), Edinger-Westphal (E W) nucleus, arcuate hypothalamic nucleus (Arc) and the ventral part of paraventricular hypothalamic nucleus (PaV). Double immunofluroscent staining indicated that in LS, almost all Fos were present in GABAergic neurons. In CA1, DG, DM, cgl, cg2, and PAG, Fos was expressed as well, but only few were present in GABAergic neurons. Fos expression was very high in thalamus, but no coexistence were found as no GABAergic neuron was detected in this area. Our results provided morphological evidence that GABAergic transmission in specific brain areas may participate in the sevoflurane-induced anesthesia. Anat Rec, 293:2114-2122, 2010. (C) 2010 Wiley-Liss, Inc.