Neuroglobins from the zebrafish Danio rerio and the pufferfish Tetraodon nigroviridis
Neuroglobins from the zebrafish Danio rerio and the pufferfish Tetraodon nigroviridis
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DOI：
10.1006/bbrc.2001.5614
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发表时间：
2001-09-21
影响因子：
3.1
通讯作者：
Burmester, T
中科院分区：
文献类型：
--
作者：
Awenius, C;Hankeln, T;Burmester, T
脑红蛋白是最近发现的一种含卟啉的呼吸蛋白，在小鼠和人脑中表达。报道了河豚和斑马鱼的cDNA全序列。此外，T.测序结果表明，B12.2、E11.0和G7.0内含子的保守性以及在5'非编码区存在另外的内含子。鱼神经球蛋白各包含159个氨基酸，并且84.3%相同。系统发育分析表明，在后生动物珠蛋白树的基础位置的神经球蛋白。与哺乳动物的蛋白质相比，鱼类神经球蛋白的氨基酸替换率（每年每个位点的替换率为0.39 × 10 - 9）估计有所增加（每年每个位点的替换率为0.93 × 10-9）。(C)北京：科学出版社.
Neuroglobin is a recently discovered respiratory, porphyrin-containing protein that is expressed in the brain of mouse and man. Here we show that neuroglobin is also present in the teleost fish. Complete cDNA sequences are reported from the pufferfish Tetraodon nigroviridis and the zebrafish Danio rerio. In addition, the neuroglobin gene of T. nigroviridis was sequenced, demonstrating the conservation of the B12.2, E11.0 and G7.0 introns plus the presence of an additional intron in the 5' noncoding region. The fish neuroglobins each comprise 159 amino acids and are 84.3% identical. Phylogenetic analyses show a basal position of the neuroglobins within the metazoan globin tree. An enhanced amino acid substitution rate was estimated for the fish neuroglobins (similar to0.93 x 10(-9) amino acid substitutions per site and year) compared with their mammalian proteins (similar to0.39 x 10-9 replacements per site and year). (C) 2001 Academic Press.