Proteomic profiles of embryonic development in the ascidian Ciona intestinalis
Proteomic profiles of embryonic development in the ascidian Ciona intestinalis
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DOI：
10.1016/j.ydbio.2008.10.038
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发表时间：
2009-01-15
影响因子：
2.7
通讯作者：
Inaba, Kazuo
中科院分区：
文献类型：
--
作者：
Nomura, Mamoru;Nakajima, Ayako;Inaba, Kazuo
我们在这里报告基于蛋白质组学的蛋白质谱的三个胚胎阶段的海鞘玻璃海鞘。二维凝胶电泳显示416，539，和695蛋白点在未受精卵，16细胞期胚胎，蝌蚪幼虫，分别。斑点模式的比较和定量分析确定了在胚胎发育过程中数量增加或减少的蛋白质。MALDI-TOF/MS的蛋白质鉴定不仅表明代谢酶和翻译延伸因子的丰度和重要性，而且还表明肌动蛋白结合蛋白和分子伴侣在海鞘发育过程中的功能重要性。卵黄原蛋白样蛋白斑点的全球变化表明该蛋白在胚胎发生过程中的翻译后修饰或蛋白水解消化。未受精卵，16细胞期胚胎和蝌蚪幼虫之间的mRNA和蛋白质水平的比较表明基因和蛋白质的非平行表达模式。海鞘为研究发育过程中的基因表达和细胞分化提供了一个很好的系统，目前的研究应该在蛋白质水平上阐明相关的分子机制。(C)2008年爱思唯尔公司All rights reserved.
We report here proteomics-based protein profiles of three embryonic stages of the ascidian Ciona intestinalis. Two-dimensional gel electrophoresis revealed 416, 539, and 695 protein spots in the unfertilized eggs, 16 cell-stage embryos, and tadpole larvae, respectively. Comparative and quantitative analyses of the spot patterns identified proteins showing an increase or decrease in amount during embryonic development. Protein identification by MALDI-TOF/MS indicated not only the abundance and importance of metabolic enzymes and translation elongation factors but also the functional importance of actin-binding proteins and molecular chaperones during ascidian development. Global changes in spots for vitellogenin-like protein suggested post-translational modification or proteolytic digestion of this protein during embryogenesis. Comparison between mRNA and protein levels among unfertilized eggs, 16 cell-stage embryos and tadpole larvae indicated nonparallel expression patterns of genes and proteins. Ascidians provide an excellent system for studying gene expression and cell differentiation during development, and the present study should shed light on the associated molecular mechanism at the protein level. (C) 2008 Elsevier Inc. All rights reserved.