Effects of establishing cell cultures and cell culture conditions on the proliferative life span of human fibroblasts isolated from different tissues and donors of different ages
Effects of establishing cell cultures and cell culture conditions on the proliferative life span of human fibroblasts isolated from different tissues and donors of different ages
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DOI：
10.1006/excr.2002.5485
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发表时间：
2002-04-01
影响因子：
3.7
通讯作者：
Allen, RG
中科院分区：
文献类型：
--
作者：
Balin, AK;Fisher, AJ;Allen, RG
众所周知，置于培养环境中的正常人细胞表现出有限的增殖能力。培养环境影响增殖寿命的程度尚不清楚。本研究评价了用于建立和维持培养物的标准程序对从胎儿和成人皮肤和肺建立的不同类型人成纤维细胞增殖寿命的影响。本研究的结果表明，建立细胞培养的程序只使用活检切片中存在的几个细胞亚群之一，大多数实验室常规使用的培养条件可以对增殖寿命测定产生显着影响。当通过在两种常用的商业培养基中生长细胞获得时，最大增殖寿命显著不同。尽管从成人皮肤建立的线对毒性的抵抗力更强，但在所有检查的线中，呼吸寿命与环境氧张力呈负相关，与播种密度直接相关。胎儿皮肤成纤维细胞的酶抗氧化防御水平远低于成人皮肤成纤维细胞中观察到的水平，但氧气对其寿命的影响是相似的。在抗氧化酶水平较低的细胞系中，高氧诱导谷胱甘肽浓度增加较大。(C)2002 Elsevier Science（美国）。
It is well known that normal human cells placed in a culture environment exhibit a limited proliferative capacity. The extent to which the culture environment influences proliferative life span is not understood. This study evaluated the effects of the standard procedures used to establish and maintain cultures on the proliferative life spans of different types of human fibroblast cells established from fetal and adult skin and lung. The results of this study demonstrate that procedures to establish cell cultures use only one of several subpopulations of cells present in biopsy pieces and that the culture conditions routinely employed by most laboratories can exert significant effects on proliferative life-span determinations. The maximum proliferative life span differed significantly when obtained by growing the cells in two commonly used commercial media. Proliferative life span was inversely related to ambient oxygen tension and directly related to seeding density in all of the lines examined although lines established from adult skin were much more resistant to toxicity. Enzymatic antioxidant defense levels of fetal skin fibroblasts were much lower than those observed in adult skin fibroblasts, but the effects of oxygen on their life spans were similar. Hyperoxia induced larger increases in glutathione concentration in cell lines with low antioxidant enzyme levels. (C) 2002 Elsevier Science (USA).