Development of Cell Chip Based on Track Detector for Examination of Biological Damages by Alpha Particles
Development of Cell Chip Based on Track Detector for Examination of Biological Damages by Alpha Particles
复制标题
开发基于跟踪探测器的细胞芯片，用于检查阿尔法粒子的生物损伤
DOI：
10.1080/18811248.2010.9720987
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发表时间：
2010
影响因子：
1.2
通讯作者：
Y. Kato and T. Iida
中科院分区：
文献类型：
--
作者：
T. Kuchimaru;F. Sato;S. Tanaka;I. Murata;Y. Kato and T. Iida
研制了一种细胞芯片，用于检测高能α粒子照射下细胞的生物损伤。采用CR-39轨迹探测器作为芯片衬底，用于识别穿过靶细胞的高能带电粒子。此外，光聚合物层的图案在空间上控制了芯片衬底上的细胞粘附性。将HeLa癌细胞培养在微图案光聚合物层上。通过这种方式，芯片上的所有细胞都通过光聚合物模式中的块号单独寻址。通过荧光试剂的活力试验和细胞增殖率的测定来检测细胞芯片的生物相容性。芯片上的HeLa癌细胞用α粒子照射，并用荧光探针分子染色，用于DNA损伤检测。CR-39底物在细胞孵育期间用碱溶液蚀刻。在显微镜下成功地观察到HeLa细胞和α轨道。证实DNA损伤对应的荧光点位于椭圆形α轨道长轴方向。
A cell chip was developed for the examination of biological damage of cells irradiated by high-energy alpha particles. A CR-39 track detector was employed as a chip substrate to identify high-energy charged particles traversing target cells. Moreover, the patterning of a photopolymer layer spatially controlled the cellular adhesiveness on the chip substrate. HeLa cancer cells were cultured on a micropatterned photopolymer layer. In this way, all the cells on the chip were individually addressed through the block number in the photopolymer pattern. The biocompatibility of the cell chip was examined through a viability test with fluophor reagent and measurement of the cell proliferation rate. HeLa cancer cells on the chip were irradiated with alpha particles and stained with a fluorescent probe molecule for DNA damage detection. The CR-39 substrate was etched by means of an alkali solution during cell incubation. The HeLa cells and alpha tracks were successfully observed by microscopy at once. It was confirmed that fluorescent spots corresponding to DNA damage were located in the direction of the major axis of oval alpha tracks.