Plate-to-plate fluorous solid-phase extraction for solution-phase parallel synthesis.
Plate-to-plate fluorous solid-phase extraction for solution-phase parallel synthesis.
复制标题
用于溶液相平行合成的板对板含氟固相萃取。
DOI：
10.1021/cc050061z
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发表时间：
2005
影响因子：
--
通讯作者：
Nagashima,Tadamichi
中科院分区：
文献类型：
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作者：
Zhang,Wei;Lu,Yimin;Nagashima,Tadamichi
采用配备有24个FluoroFlashcartridges的市售Argonaut VacMaster-96板对板固相萃取（SPE）站来平行纯化氟反应混合物。每个装有3 g氟硅胶的柱能够产生高达100 mg的纯化小分子。24孔接收板具有可在Genevac真空离心机中直接浓缩的标准占地面积。重要的问题，如样品加载，产品交叉污染，盒重复使用，和再现性进行了研究。固相萃取系统已被证明在三个小的图书馆，涉及胺清除反应与氟靛红酸酐，酰胺偶联反应与2-氯-4，6-双[（全氟己基）丙氧基]-1，3，5-三嗪（氟CDMT），和酰胺偶联反应与新开发的氟Mukaiyama缩合试剂的纯化。
A commercially available Argonaut VacMaster-96 plate-to-plate solid-phase extraction (SPE) station equipped with 24 FluoroFlashcartridges is employed for parallel purification of fluorous reaction mixtures. Each cartridge charged with 3 g of fluorous silica gel has the capability to produce up to 100 mg of purified small molecules. The 24-well receiving plate has a standard footprint that can be directly concentrated in a Genevac vacuum centrifuge. Important issues such as sample loading, product cross-contamination, cartridge reuse, and reproducibility are investigated. The SPE system has been demonstrated in the purification of three small libraries that were produced involving amine scavenging reactions with fluorous isatoic anhydride, amide coupling reactions with 2-chloro-4,6-bis[(perfluorohexyl)propyloxy]-1,3,5-triazine (fluorous CDMT), and amide coupling reactions with a newly developed fluorous Mukaiyama condensation reagent.