PROLONGATION OF ISLET XENOGRAFT SURVIVAL BY IN VITRO CULTURE OF RAT MEGAISLETS IN 95% O21
PROLONGATION OF ISLET XENOGRAFT SURVIVAL BY IN VITRO CULTURE OF RAT MEGAISLETS IN 95% O21
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延长%20OF%20ISLET%20XENOGRAFT%20SURVIVAL%20BY%20IN%20VITRO%20CULTURE%20OF%20RAT%20MEGAISLETS%20IN%2095%%20O21
DOI：
10.1097/00007890-198206000-00004
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发表时间：
1982
期刊：
影响因子：
6.2
通讯作者：
J. Davie
中科院分区：
文献类型：
--
作者：
P. Lacy;E. Finke;C. Janney;J. Davie
单个大鼠胰岛可以聚集成单一的巨胰岛在体外和巨胰岛保持形态和功能完整后，在95%O2和5%CO2的存在下培养7天。培养的大鼠巨胰岛移植到糖尿病小鼠的肾包膜下，在受体中产生了正常的胰岛素，并且异种移植物的存活时间通过7天暴露于高氧张力而显著延长。单次注射小鼠和大鼠淋巴细胞的抗淋巴细胞血清到接受培养的巨胰岛的受体中，移植后70天移植物的存活百分比没有进一步增加。在移植后60和90天，在培养的阴性细胞的异种移植物周围存在类水母聚集体。这种淋巴反应不干扰异种移植物的功能，因为受体血糖正常，并且移植物的移除导致迅速恢复到糖尿病状态。培养的大鼠巨胰岛的门静脉内和脾内移植物不能存活，只要移植到肾包膜下的异种移植物。肾被膜下位点显然提供了一些免疫优势，延迟急性排斥反应，因为移植的个人，新鲜的大鼠胰岛存活的时间为2倍，在肾包膜下相比，门静脉内移植的新鲜大鼠胰岛。
Individual rat islets could be aggregated into single megaislets in vitro and the megaislets remained morphologically and functionally intact after a 7-day period of culture in the presence of 95% O2 and 5% CO2. Cultured rat megaislets transplanted beneath the renal capsule of diabetic mice produced normoglycemia in the recipients and the survival of the xenografts was markedly prolonged by the 7-day exposure to a high oxygen tension. A single injection of antilymphocyte sera to mouse and rat lymphocytes into the recipients receiving cultured megaislets did not produce a further increase in the percentage of survival of the grafts at 70 days after transplantation. Lymphoid aggregates were present around xenografts of cultured negaislets at 60 and 90 days after transplantation. This lymphoid reaction did not interfere with the function of the xenografts since the recipients were normoglycemic and removal of the grafts resulted in a rapid return to a diabetic state. Intraportal and intrasplenic transplants of cultured rat megaislets did not survive as long as the xenografts of megaislets transplanted beneath the renal capsule. The renal subcapsule site apparently provided some immunological advantage for delaying acute rejection since transplants of individual, fresh rat islets survived for twice as long under the renal capsule as compared with intraportal transplants of fresh rat islets.