IMAGING SOFT SAMPLES WITH THE ATOMIC-FORCE MICROSCOPE - GELATIN IN WATER AND PROPANOL
IMAGING SOFT SAMPLES WITH THE ATOMIC-FORCE MICROSCOPE - GELATIN IN WATER AND PROPANOL
复制标题
DOI：
10.1016/s0006-3495(95)79897-6
复制
发表时间：
1995-07-01
影响因子：
3.4
通讯作者：
HANSMA, PK
中科院分区：
文献类型：
--
作者：
RADMACHER, M;FRITZ, M;HANSMA, PK
我们用原子力显微镜（AFM）在水、丙醇和这两种液体的混合物中对涂有薄明胶薄膜的云母进行了成像。弹性模量（杨氏模量）可以根据丙醇与水的比例从20 kPa调整到0.1 GPa以上。在纯丙醇中分辨率最好，约为20 nm，而在较软的样品中分辨率变差。通过考虑由AFM尖端引起的明胶的弹性压痕，可以理解分辨率的退化。这种压痕变得更大，因此接触面积变得更大的软样品。因此，本研究可用于估计AFM对其他软样品（如细胞）的分辨率。非破坏性成像只能通过在力下成像
We have imaged mica coated with thin gelatin films in water, propanol, and mixtures of these two liquids by atomic force microscopy (AFM). The elastic modulus (Young's modulus) can be tuned from 20 kPa to more than 0.1 GPa depending on the ratio of propanol to water. The resolution is best in pure propanol, on the order of 20 nm, and becomes worse for the softer samples. The degradation in resolution can be understood by considering the elastic indentation of the gelatin caused by the AFM tip. This indentation becomes larger and thus the contact area becomes larger the softer the sample is. Therefore this study may be used to estimate the resolution to be expected with an AFM on other soft samples, such as cells. Nondestructive imaging was possible only by imaging at forces