Differential G-protein expression during B- and T-cell development
Differential G-protein expression during B- and T-cell development
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DOI：
10.1046/j.1365-2567.1997.00196.x
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发表时间：
1997-04-01
期刊：
影响因子：
6.4
通讯作者：
Harnett, MM
中科院分区：
文献类型：
--
作者：
Grant, KR;Harnett, W;Harnett, MM
迄今为止，人们对 B 细胞和 T 细胞发育的分子机制知之甚少。然而，由于 G 蛋白调节多种生物反应，包括生长、增殖和分化，我们研究了 B 细胞和 T 细胞发育过程中 G 蛋白的差异表达，目的是识别淋巴细胞成熟过程中涉及的关键信号。在整个淋巴发育过程中发现了 Gs、i 和 q 家族的 β(1/2) 和 α 亚基的差异表达。最引人注目的是，G α(i1) 和 G α(11) 在未成熟、未成熟和成熟 B 细胞、胸腺细胞或成熟 T 细胞中表达非常弱，或不表达，但在成熟 B 类淋巴母细胞系中强烈诱导，其中一些细胞系已用作生发中心 B 细胞的模型，表明这些 G 蛋白的表达可能与 B 细胞发育的后期阶段相关。相比之下，G α(16) 表达在 T 细胞和前 B 细胞中最高，并随着 B 细胞成熟而逐渐下降。这些发现表明，G 蛋白及其调节的信号，例如离子通道和/或腺苷酸环化酶 (G alpha(s/i)) 和磷脂酶 C（G beta gamma 和 G alpha(11/16)）在淋巴细胞中以成熟和谱系依赖性方式受到差异性调节。
The molecular mechanisms underlying B- and T-cell development are, as yet, poorly understood. However, as G proteins regulate a diverse range of biological responses including growth, proliferation and differentiation, we have investigated differential expression of G proteins during B- and T-cell development with the aim of identifying key signals involved in lymphocyte maturation. Differential expression of beta(1/2) and alpha-subunits of the Gs-, i- and q-families was found throughout lymphoid development. Most strikingly, G alpha(i1) and G alpha(11) were very weakly, or not expressed in pre-, immature and mature B cells, thymocytes or mature T cells, but strongly induced in mature B-lymphoblastoid cell lines, some of which have been used as models of germinal centre B cells, suggesting that expression of these G proteins may correlate with the later stages of B-cell development. In contrast, G alpha(16) expression was highest in T cells and pre-B cells and progressively declined with B-cell maturation. These findings suggest that G proteins, and the signals they regulate, such as ion channels and/or adenylate cyclase (G alpha(s/i)) and phospholipase C (G beta gamma and G alpha(11/16)) are differentially regulated in lymphoid cells in a maturation- and lineage-dependent manner.