Assessment of immunity to mycobacterial infection with luciferase reporter constructs
Assessment of immunity to mycobacterial infection with luciferase reporter constructs
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DOI：
10.1128/iai.67.9.4586-4593.1999
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发表时间：
1999-09-01
影响因子：
3.1
通讯作者：
Young, DB
中科院分区：
文献类型：
--
作者：
Snewin, VA;Gares, MP;Young, DB
对分枝杆菌感染的保护性免疫还不完全清楚，但可能涉及多种细胞类型和细胞因子的协调相互作用。为了开发可能提供保护性免疫替代措施的测定法，黑麦研究了使用携带荧光素酶报告酶的重组分枝杆菌来评估模型系统中抗分枝杆菌免疫的有效性。研究表明，发光测量可以提供一种快速、简单的替代CFU计数的方法，作为监测分支杆菌活力的手段。我们描述了结核分枝杆菌的荧光素酶报告菌株的优化，并证明其应用于组织培养中的分枝杆菌与宿主细胞的相互作用的研究和在小鼠模型中的疫苗效力的快速评估。
Protective immunity to mycobacterial infection is incompletely understood but probably involves the coordinated interaction of multiple cell types and cytokines, With the aim of developing assays that might provide a surrogate measure of protective immunity, rye have investigated the use of recombinant mycobacteria carrying luciferase reporter enzymes to assess the effectiveness of antimycobacterial immunity in model systems. Measurement of luminescence was shown to provide a rapid and simple alternative to the counting of CFU as a means of monitoring mycobacterial viability. We describe optimization of a luciferase reporter strain of Mycobacterium tuberculosis and demonstrate its application for the study of mycobacterial interactions with host cells in tissue culture and the rapid assessment of vaccine efficacy in a murine model.