Lignin-carbohydrate complexes: Intestinal immune system modulating ingredients in Kampo (Japanese herbal) medicine, Juzen-Taiho-To
Lignin-carbohydrate complexes: Intestinal immune system modulating ingredients in Kampo (Japanese herbal) medicine, Juzen-Taiho-To
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DOI：
10.1055/s-2000-11116
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发表时间：
2000-02-01
期刊：
影响因子：
2.7
通讯作者：
Yamada, H
中科院分区：
文献类型：
--
作者：
Kiyohara, H;Matsumoto, T;Yamada, H
已发现日本草药（汉方）药物Juzen-Taiho-To的提取物制剂（TJ-48）对由派伊尔斑细胞介导的骨髓细胞增殖显示出增强活性（肠免疫系统调节活性）（1）。当TJ-48通过甲醇和水提取、乙醇沉淀和透析进行分级时，水溶性可透析级分（F-3）和多糖级分（F-5）均显示出显著的体外肠道免疫系统调节活性;其他级分没有活性。口服给药F-3（150 mg/kg）也显示了C3 H/HeJ小鼠肠道免疫系统调节活性。通过凝胶过滤从F-3中纯化两种活性物质。化学分析表明，活性物质包括木质素以及主要由阿拉伯糖、半乳糖、葡萄糖和半乳糖醛酸组成的碳水化合物。活性显着降低，不仅由NaIO 4氧化，但也由NaClO 2处理。标准木质素显示无活性。活性物质中的碳水化合物和木质素通过苯基-琼脂糖凝胶疏水相互作用层析共洗脱，并且即使在疏水相互作用层析之后，物质的刺激效果和木质素含量也没有改变。这些结果表明，木质素-碳水化合物复合物参与TJ-48对肠道免疫系统的调节。
An extract preparation (TJ-48) of a Japanese herbal (Kampo) medicine, Juzen-Taiho-To has been found to show an enhancing activity on proliferation of bone marrow cells mediated by Peyer's patch cells (intestinal immune system modulating activity) (1). When TJ-48 was fractionated by MeOH- and water-extractions, EtOH precipitation and dialysis, water-soluble dialyzable (F-3) and polysaccharide fractions (F-5) both showed a significant intestinal immune system modulating activity in vitro; other fractions had no activity. Oral administration of F-3 (150 mg/kg) also showed the intestinal immune system modulating activity in C3H/HeJ mice. Two active substances were purified by gel filtration from F-3. Chemical analysis indicated that the active substances comprised lignin as well as a carbohydrate consisting mainly of arabinose, galactose, glucose and galacturonic acid. Activity was significantly reduced not only by NaIO4 oxidation but also by NaClO2 treatment. Standard lignin showed no activity. Carbohydrate and lignin in the active substances were co-eluted by hydrophobic-interaction chromatography on phenyl-Sepharose, and the stimulating effect and lignin content of the substances did not change even after the hydrophobic-interaction chromatography. These results suggest that lignin-carbohydrate complexes are involved in intestinal immune system modulation by TJ-48.