Interaction of tsg101 with Marburg virus VP40 depends on the PPPY motif, but not the PT/SAP motif as in the case of Ebola virus, and tsg101 plays a critical role in the budding of Marburg virus-like particles induced by VP40, NP, and GP
Interaction of tsg101 with Marburg virus VP40 depends on the PPPY motif, but not the PT/SAP motif as in the case of Ebola virus, and tsg101 plays a critical role in the budding of Marburg virus-like particles induced by VP40, NP, and GP
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DOI：
10.1128/jvi.02829-06
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发表时间：
2007-05-01
影响因子：
5.4
通讯作者：
Yasuda, Jiro
中科院分区：
文献类型：
--
作者：
Urata, Shuzo;Noda, Takeshi;Yasuda, Jiro
马尔堡病毒（MARV）VP 40是一种基质蛋白，可以以病毒样颗粒（VLP）的形式从哺乳动物细胞中释放，并含有PPPY序列，该序列是L结构域基序。在这里，我们证明了PPPY基序是重要的VP 40诱导的VLP出芽和VLP生产显着增强NP和GP的共表达。我们表明，Tsg 101与VP 40的相互作用取决于PPPY基序的存在，而不是像埃博拉病毒那样取决于PT/SAP基序的存在，并且在VLP萌芽中发挥着重要作用。这些发现为MARV出芽机制提供了新的见解。
Marburg virus (MARV) VP40 is a matrix protein that can be released from mammalian cells in the form of virus-like particles (VLPs) and contains the PPPY sequence, which is an L-domain motif. Here, we demonstrate that the PPPY motif is important for VP40-induced VLP budding and that VLP production is significantly enhanced by coexpression of NP and GP. We show that Tsg101 interacts with VP40 depending on the presence of the PPPY motif, but not the PT/SAP motif as in the case of Ebola virus, and plays an important role in VLP budding. These findings provide new insights into the mechanism of MARV budding.