AFM visualization of cortical filaments/network under cell-bound membrane vesicles.
AFM visualization of cortical filaments/network under cell-bound membrane vesicles.
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DOI：
10.1016/j.bbamem.2015.06.025
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发表时间：
2015-10
期刊：
影响因子：
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通讯作者：
Xiaojun Zhang;Qisheng Tang;Li Wu;Jie Huang;Yong Chen
中科院分区：
文献类型：
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作者：
Xiaojun Zhang;Qisheng Tang;Li Wu;Jie Huang;Yong Chen
虽然循环/质膜囊泡已被广泛表征，但由于缺乏有效的方法，人们对细胞结合的膜囊泡（包括其形状和与细胞内细胞骨架的相关性）知之甚少。在这项研究中，我们专注于细胞结合膜囊泡和单个囊泡衍生的坑内皮细胞上使用共聚焦显微镜和原子力显微镜（AFM）。我们首次发现，细胞结合膜囊泡是半球形的，并且肌动蛋白皮质丝/网络位于囊泡的胞质开口处，而不是紧密地附着在囊泡膜的内侧。细胞结合膜囊泡的这种结构可能有利于它们在细胞质膜中的移动或从细胞质膜释放，这是由于更少的膜-细胞骨架偶联被破坏，因此可能使能量消耗和时间使用最小化。进一步的研究表明，TNF-α激活诱导细胞结合囊泡的平均数量/大小显著增加，并在细胞结合囊泡的胞质开口处局部破坏肌动蛋白网络。
While circulating/plasma membrane vesicles have been extensively characterized, due to the lack of effective methods cell-bound membrane vesicles are poorly understood including their shape and correlation with the intracellular cytoskeleton. In this study, we focused on cell-bound membrane vesicles and individual vesicle-derived pits on endothelial cells by using confocal microscopy and atomic force microscopy (AFM). For the first time, we found that cell-bound membrane vesicles are hemisphere-shaped and that the actin cortical filaments/network lies at the cytosolic opening of a vesicle instead of being closely attached to the inner side of the vesicle membrane. This structure of cell-bound membrane vesicles may be beneficial to their movement in, or release from, the plasma membrane of cells due to less membrane–cytoskeleton coupling to be broken therefore probably minimizing energy consumption and time usage. Further study indicates that TNF-α activation induced a significant increase in average number/size of cell-bound vesicles and the local disruption of the actin network at the cytosolic opening of cell-bound vesicles.