Cell tracking. Principles and applications
Cell tracking. Principles and applications
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DOI：
10.1007/s00117-006-1449-5
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发表时间：
2007-01-01
期刊：
影响因子：
--
通讯作者：
Weissleder, R.
中科院分区：
文献类型：
--
作者：
Grimm, J.;Kircher, M. F.;Weissleder, R.
目前正在探索基于细胞的疗法如干细胞疗法或过继免疫疗法作为多种疾病如帕金森病、糖尿病或癌症的潜在治疗。然而，过继转移细胞的定量和定性评价对于监测治疗效率是必不可少的。目前的方法主要分析来自外周血的转移细胞，这不能评估转移的细胞是否真的回家并留在靶组织中。使用细胞标记方法，例如直接标记或用标记基因转染结合各种成像方式（MRI、光学或核成像），可以在体内实时跟踪标记的细胞，并且可以准确地监测和定量它们的体内积累以及功能。这种方法通常被称为“细胞追踪”或“细胞运输”，也被应用于基础生物科学，例如评估有助于转移的基因。这篇评论的重点是这种有前途的方法的原则，并解释了各种方法，突出最近的例子。
Cell based therapies such as stem cell therapies or adoptive immunotherapies are currently being explored as a potential treatment for a variety of diseases such as Parkinson's disease, diabetes or cancer. However, quantitative and qualitative evaluation of adoptively transferred cells is indispensable for monitoring the efficiency of the treatment. Current approaches mostly analyze transferred cells from peripheral blood, which cannot assess whether transferred cells actually home to and stay in the targeted tissue. Using cell-labeling methods such as direct labeling or transfection with a marker gene in conjunction with various imaging modalities (MRI, optical or nuclear imaging), labeled cells can be followed in vivo in real-time, and their accumulation as well as function in vivo can be monitored and quantified accurately. This method is usually referred to as "cell tracking" or "cell trafficking" and is also being applied in basic biological sciences, exemplified in the evaluation of genes contributing to metastasis. This review focuses on principles of this promising methodology and explains various approaches by highlighting recent examples.