Polypyrrole Hydrogel with Near-infrared Light-driven Nitric Oxide Release and Photothermal Activities for Rapid Synergistic Sterilization and Infected Wound Therapy
Polypyrrole Hydrogel with Near-infrared Light-driven Nitric Oxide Release and Photothermal Activities for Rapid Synergistic Sterilization and Infected Wound Therapy
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DOI：
10.1016/j.colsurfa.2023.132411
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发表时间：
2023-09
期刊：
影响因子：
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通讯作者：
Min Wang;Jie Hao;Wen-tai Guo;Tao Huang;Changqing Miao;Luchang Yin;Hongjie Ji;Fei Gao;Baolong Zhou
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文献类型：
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作者：
Min Wang;Jie Hao;Wen-tai Guo;Tao Huang;Changqing Miao;Luchang Yin;Hongjie Ji;Fei Gao;Baolong Zhou
随着细菌感染的日益严重，迫切需要开发低成本、易获得、杀菌效率高的光疗药物。本文中，广谱抗微生物剂（即，将一氧化氮（NO）热敏供体（N，N′-二仲丁基-N，N ′-二亚硝基-1，4-苯二胺，BNN 6）与磺化二钼-salen交联的三维聚吡咯（PP-Mo-NO）水凝胶组装，合成了具有近红外激光（NIR）驱动释放NO能力的PP-Mo-NO。大肠杆菌）和革兰氏阳性金黄色葡萄球菌（S. aureus)具有良好的抗菌活性。在808 nm激光照射下，PP-Mo-NO不仅表现出优异的光热效率（79.88%），而且具有可精确控制的NO释放能力，产生氧化/亚硝化应激。在10 min的辐照时间内（1.0Wcm-2），PP-Mo-NO具有协同增强的光热（PTT）/NO协同抗菌活性，其对大肠杆菌和铜绿假单胞菌的杀菌率均超过99.1%。大肠杆菌和S.金黄色葡萄球菌的浓度相对较低（100 µg mL−1）。同时，体内实验证明PP-Mo-NO具有良好的应用潜力，可有效杀灭感染部位的细菌，修复开放性感染创面，加速组织重建。
Developing low-cost and easy-accessible phototherapy agents with a high sterilization efficiency is urgently needed tackling the increasingly dangerous of bacterial infections. Herein, a broad-spectrum antimicrobial agent (i.e.,PP-Mo-NO) with near-infrared laser (NIR) driven nitric oxide (NO) release capacity derived from the assemble of a heat-sensitive NO donor (N, N′-di-sec-butyl-N, N′-dinitroso-1, 4-phenylenediamine, BNN6) with freeze-dried three-dimensional polypyrrole hydrogel crosslinked by the sulfonated dimolybdenum-salen (PP-Mo), was synthesized for the rapid and effective eradicating of Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus). Upon 808 nm laser irradiation, PP-Mo-NO showed not only superb photothermal efficacy (79.88 %), but also precisely controllable NO release capacity, generating oxidative/nitrosative stress. Within 10 mins of irradiation (1.0 W cm−2), PP-Mo-NO with co-amplified photothermal (PTT)/NO synergetic antibacterial activity presented sterilization rates beyond 99.1% against both theE. coliandS. aureusat a relatively low concentration (100 µg mL−1). Meanwhile, in vivo experiments demonstrated the great application potential of PP-Mo-NO which could effectively kill bacteria at the site of infection, and repair the open infectious wounds, thereby accelerating the tissue reconstruction.