BLOODMEAL IDENTIFICATION BY DIRECT ENZYME-LINKED IMMUNOSORBENT-ASSAY (ELISA), TESTED ON ANOPHELES (DIPTERA, CULICIDAE) IN KENYA
BLOODMEAL IDENTIFICATION BY DIRECT ENZYME-LINKED IMMUNOSORBENT-ASSAY (ELISA), TESTED ON ANOPHELES (DIPTERA, CULICIDAE) IN KENYA
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DOI：
10.1093/jmedent/25.1.9
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发表时间：
1988-01-01
影响因子：
2.1
通讯作者：
KOECH, DK
中科院分区：
文献类型：
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作者：
BEIER, JC;PERKINS, PV;KOECH, DK
建立了一种直接酶联免疫吸附试验（ELISA），用于7种宿主的血粉鉴定。使用市售试剂；试验仅需4.5小时即可完成。干蚊在进食32小时后可检出血粉，冻蚊则可检出血粉23小时。采用抗人过氧化物酶偶联物和抗牛磷酸酶偶联物两步法，在同一微滴板孔中检测一只蚊子对两种宿主的感染。该方法应用于冈比亚按蚊。以及在肯尼亚西部的小屋里收集的A. funestus Giles；在4338份血餐中，有94%被鉴定为人（88%）、牛（4%）或人牛混合（2%）。此外，还开发了一种系统，可以同时用血粉酶联免疫吸附试验和疟疾孢子虫酶联免疫吸附试验检测一只蚊子。
A direct enzyme-linked immunosorbent assay (ELISA) was developed for bloodmeal identification of seven hosts. Commercially available reagents are used; the test can be completed in only 4.5 h. Blood meals can be detected up to 32 h after feeding for dried mosquitoes and up to 23 h for frozen mosquitoes. A two-step procedure, using anti-human peroxidase conjugate and antibovine phosphatase conjugate, was developed to test a single mosquito for two hosts in the same microtiter plate well. The assay was appplied to Anopheles gambiae Giles, s. lat. and A. funestus Giles collected inside huts in western Kenya; 94% of 4,338 blood meals were identified as either human (88%), cow (4%), or mixed human-cow (2%). Additionally, a system was developed whereby a single mosquito could be tested by both the blood meal ELISA and the malaria sporozoite ELISA.