Antioxidative, antimutagenic, and anticarcinogenic activities of rice bran extracts in chemical and cell assays
Antioxidative, antimutagenic, and anticarcinogenic activities of rice bran extracts in chemical and cell assays
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DOI：
10.1021/jf0490293
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发表时间：
2005-02-09
影响因子：
6.1
通讯作者：
Friedman, M
中科院分区：
文献类型：
--
作者：
Nam, SH;Choi, SP;Friedman, M
乙醇水（70：30 v/v）从两种黑紫色色素的种子中取出的米糠提取物（Sanhaehyanghyulla和水原415）和一个无色素的本研究以化学分析法及哺乳动物细胞法，评估竹中糙米品种之抗氧化、抗肿瘤及抗癌活性（人白血病HL-60、绒猴B类淋巴母细胞B 95 -8和中国仓鼠V79肺细胞）通过以下试验：抑制黄嘌呤氧化酶活性;螯合亚铁离子;还原铁氰化钾;清除超氧阴离子、羟基自由基和细胞内过氧化物;抑制4-硝基喹啉N-氧化物诱导诱变;和抑制佛波酯诱导的肿瘤促进。色素水稻种子的提取物在所有试验中的活性普遍高于非色素品种的提取物。这些结果表明，色素水稻品种的麸皮可能提供了一种新的天然抗氧化剂和抗癌剂的来源，这种具有高抗氧化潜力的水稻品种也提供了一种遗传资源，用于开发新的，改良的水稻品种，这可能会提高以大米为基础的饮食的营养和医疗价值。
Ethanol-water (70:30 v/v) extracts from rice brans removed from seeds of two blackish-purple pigmented (Sanhaehyanghyulla and Suwon 415) and one nonpigmented (Chuchung) brown rice cultivars were evaluated for antioxidative, anti-tumor-promoting, and anticarcinogenic activities in chemical assays and in mammalian cells (human leukemia HL-60, marmoset B lymphoblastoid B95-8, and Chinese hamster V79 lung cells) by the following tests: inhibition of xanthine oxidase activity; chelation of ferrous ions; reduction of potassium ferricyanide; scavenging of superoxide anions, hydroxyl radicals, and intracellular peroxides; inhibition of 4-nitroquinoline N-oxide-induced mutagenesis; and inhibition of phorbol ester-induced tumor promotion. The extracts from the pigmented rice seeds had generally higher activities in all tests than did the extract from the nonpigmented variety. The results suggest that brans from pigmented rice varieties may provide a source of new natural antioxidants and anticarcinogens and that such rice cultivars with high antioxidative potential also provide a genetic resource for the development of new, improved rice cultivars that may make it possible to enhance both the nutritional and medical value of rice-based diets.