Molecular cloning, biological effect, and tissue distribution of interleukin‐8 protein in mandarin fish (Siniperca chuasti) upon Flavobacterium columnare infection
Molecular cloning, biological effect, and tissue distribution of interleukin‐8 protein in mandarin fish (Siniperca chuasti) upon Flavobacterium columnare infection
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DOI：
10.1016/j.fsi.2017.05.016
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发表时间：
2017-07
影响因子：
4.7
通讯作者：
G. Wang;M. Wang;Xiao Wen Zhang;M. Chang;H. Xie;P. Nie
中科院分区：
文献类型：
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作者：
G. Wang;M. Wang;Xiao Wen Zhang;M. Chang;H. Xie;P. Nie
白细胞介素-8（IL-8）是一种CXC型趋化因子，通过募集中性粒细胞在哺乳动物的急性炎症中发挥关键作用。本研究克隆了鳜鱼IL-8的开放阅读框架（ORF），编码99个氨基酸，并对其在炎症中的功能进行了初步研究。IL-8含有四个保守的半胱氨酸残基，其中前两个形成CXC特征基序。桂鱼IL-8基因组序列由4个外显子和3个内含子组成，具有典型的CXC趋化因子基因结构。具有生物活性的重组IL-8（rIL-8）在体外对头肾白细胞具有趋化作用，并激活炎性基因IL-8和IL-1β的转录。腹腔感染致病菌柱状黄杆菌G4后，桂鱼体肾和头肾中IL-8的稳态蛋白水平上调。这些结果表明，IL-8是一种功能性的CXC趋化因子，在细菌感染的炎症反应中起着关键作用。
Interleukin-8 (IL-8), a CXC-type chemokine, plays a key role in acute inflammation by recruiting neutrophils in mammals. In the present study, the open reading frame (ORF) ofIL-8, encoding 99 amino acids was cloned in mandarin fish, and its function in inflammation was investigated. The IL-8 contains four conserved cysteine residues, with the first two forming the CXC signature motif. The genomic sequence of mandarin fishIL-8 has four exons and three introns, a typical gene organization of the CXC chemokine. Bioactive recombinant IL-8 (rIL-8) exhibited a chemotactic effect on head kidney leukocytesin vitro, and activates the transcription of the inflammatory genes,IL-8 andIL-1β. When mandarin fish was challenged intraperitoneally with the pathogenic bacteriumFlavobacterium columnareG4, the steady-state protein level of IL-8 was up-regulated in trunk kidney and head kidney. These results suggest that IL-8 is a functional CXC chemokine in mandarin fish, and plays a key role in the inflammatory responses towards bacterial infection.