Silver(I)-imidazole cyclophane gem-diol complexes encapsulated by electrospun tecophilic nanofibers: Formation of nanosilver particles and antimicrobial activity
Silver(I)-imidazole cyclophane gem-diol complexes encapsulated by electrospun tecophilic nanofibers: Formation of nanosilver particles and antimicrobial activity
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DOI：
10.1021/ja040226s
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发表时间：
2005-02-23
影响因子：
15
通讯作者：
Youngs, WJ
中科院分区：
文献类型：
--
作者：
Melaiye, A;Sun, ZH;Youngs, WJ
合成了银咪唑环番偕二醇配合物3 [Ag_2C_36N_10O_4]（2+）_2（x）（-），其中x = OH ~-或CO ~（3 2-）。最小抑制浓度试验表明，3的水溶液形式作为抗生素的有效性比具有大约相同量的银的0.5%AgNO3低2倍。通过静电纺丝将3包封到Tecophilic聚合物中，得到纳米纤维毡，从而提高了3的抗菌活性。纤维毡释放出纳米银颗粒，这反过来又在很长一段时间内维持了毡的抗微生物活性。包封后3的杀菌率大大提高，且银用量大大减少。含有75%3和25%Tecophilic的3的纤维垫中所含的银的量比0.5%AgNO3中的低8倍，比1%磺胺嘧啶银乳膏中的低5倍。发现纤维垫可杀死S。金黄色葡萄球菌，与0.5%AgNO3相同的速率，在琼脂平板上没有菌落，比磺胺嘧啶银乳膏快约6倍。发现银垫对大肠杆菌、铜绿假单胞菌、S.金黄色葡萄球菌C. albicans、A.尼日尔和S.啤酒。用透射电子显微镜和扫描电子显微镜对纤维毡进行了表征。通过对大鼠静脉给药评估配体（咪唑环蕃偕二醇二氯化物）的急性毒性，LID 50为100 mg/kg大鼠。
Silver(I)-imidazole cyclophane gem-diol complex, 3 [Ag2C36N10O4](2+)2(x)(-), where x = OH- or CO32-, was synthesized and well characterized. The minimum inhibition concentration tests showed that the aqueous form of 3 is 2 times less effective as an antibiotic than 0.5% AgNO3, with about the same amount of silver. The antimicrobial activity of 3 was enhanced when encapsulated into Tecophilic polymer by electrospinning to obtain mats made of nano-fibers. The fiber mats released nanosilver particles, which in turn sustained the antimicrobial activity of the mats over a long period of time. The rate of bactericidal activity of 3 was greatly improved by encapsulation, and the amount of silver used was much reduced. The amount of silver contained in the fiber mat of 3, with 75% of 3 and 25% Tecophilic, was 8 times less than that in 0.5% AgNO3 and 5 times lower than that in silver sulfadiazine cream 1%. The fiber mat was found to kill S. aureus at the same rate as 0.5% AgNO3, with zero colonies on an agar plate, and about 6 times faster than silver sulfadiazine cream. The silver mats were found effective against E coli, P. aeruginosa, S. aureus, C. albicans, A. niger, and S. cerevisiae. Transmission electron microscopy and scanning electron microscopy were used to characterize the fiber mats. The acute toxicity of the ligand (imidazolium cyclophane gem-diol dichloride) was assessed by intravenous administration to rats, with an LID 50 of 100 mg/kg of rat.