Sox-2 is expressed by neural progenitors and astroglia in the adult rat brain
Sox-2 is expressed by neural progenitors and astroglia in the adult rat brain
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DOI：
10.1016/j.neulet.2004.07.035
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发表时间：
2004-10-07
影响因子：
2.5
通讯作者：
Eriksson, PS
中科院分区：
文献类型：
--
作者：
Komitova, M;Eriksson, PS
Sox-2 是一种转录因子，由胚胎神经上皮的自我更新和多能干细胞表达。然而，人们对成人大脑中 Sox-2 的表达知之甚少，因此我们使用免疫组织化学来检查其分布以及与特定细胞标记物的共定位。我们发现，Sox-2通过成年大鼠大脑神经源性区域、前脑室下区和海马齿状回颗粒下区活跃分裂的神经祖细胞表达。表达未成熟神经元标志物的细胞基本上是 Sox-2 免疫阴性的。还发现Sox-2由神经胶质纤维酸性蛋白免疫阳性星形胶质细胞表达，广泛分布于脑实质中。鉴于多项研究已经确定了成人大脑中特殊类型星形胶质细胞的神经发生能力，实质星形胶质细胞中 Sox-2 表达的发现具有潜在的意义。我们得出的结论是，Sox-2 可能与适当的细胞特异性标记物结合，构成一个有用的标记物，用于研究成人大脑中神经祖细胞区室的体内动力学。 (C) 2004 Elsevier Ireland Ltd. 保留所有权利。
Sox-2 is a transcription factor that is expressed by self-renewing and multipotent stem cells of the embryonic neuroepithelium. Very little is however known about Sox-2 expression in the adult brain and therefore we used immunohistochemistry to examine its distribution and co-localization with specific cell markers. We found that Sox-2 was expressed by actively dividing neural progenitor cells in the neurogenic regions in the adult rat brain, the subventricular zone of the forebrain and the subgranular zone of the dentate gyrus in the hippocampus. Cells expressing immature neuronal markers were essentially Sox-2 immunonegative. Sox-2 was also found to be expressed by glial fibrillary acidic protein immunopositive astroglia, widely distributed in the brain parenchyma. Given the fact that several studies have established the neurogenic capacity of a specialized type of astroglia in the adult brain, the findings of Sox-2 expression in parenchymal astroglia are of potential interest. We conclude that Sox-2 might, in combination with appropriate cell-specific markers, constitute a useful marker to study the in vivo dynamics of the neural progenitor cell compartment also in the adult brain. (C) 2004 Elsevier Ireland Ltd. All rights reserved.