Surface modified cellulose scaffolds for tissue engineering
Surface modified cellulose scaffolds for tissue engineering
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DOI：
10.1007/s10570-016-1111-y
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发表时间：
2017-01-01
期刊：
影响因子：
5.7
通讯作者：
Sharma, Ram I.
中科院分区：
文献类型：
--
作者：
Courtenay, James C.;Johns, Marcus A.;Sharma, Ram I.
我们报告了纤维素在材料表面不使用基质配体的情况下支持细胞的能力，从而创建了用于细胞和材料组织工程的双组分系统。从含有醋杆菌生物体的培养基中生长的细菌纤维素片用甘氨酰三甲基氯化铵化学改性或在溴化钠和2，2，6，6-四甲基吡啶1-氧基自由基存在下用次氯酸钠氧化以分别引入正电荷或负电荷。这种改性过程没有降低散装材料的机械性能，但接枝的带正电荷的部分的纤维素表面（阳离子纤维素）增加了70%的细胞附着相比，未改性的纤维素，而带负电荷的，氧化的纤维素膜（阴离子纤维素），显示出低水平的细胞附着相比，未改性的纤维素。仅需要最低水平的阳离子表面衍生（约3%的取代度）即可增加细胞附着，并且不需要介导蛋白质。细胞粘附研究显示出与附着研究相同的趋势，而平均细胞面积和纵横比在阳离子表面上最高。总的来说，我们证明了带正电荷的细菌纤维素在不存在用于细胞附着的蛋白质的情况下在组织工程中的效用。
We report the ability of cellulose to support cells without the use of matrix ligands on the surface of the material, thus creating a two-component system for tissue engineering of cells and materials. Sheets of bacterial cellulose, grown from a culture medium containing Acetobacter organism were chemically modified with glycidyltrimethylammonium chloride or by oxidation with sodium hypochlorite in the presence of sodium bromide and 2,2,6,6-tetramethylpipiridine 1-oxyl radical to introduce a positive, or negative, charge, respectively. This modification process did not degrade the mechanical properties of the bulk material, but grafting of a positively charged moiety to the cellulose surface (cationic cellulose) increased cell attachment by 70% compared to unmodified cellulose, while negatively charged, oxidised cellulose films (anionic cellulose), showed low levels of cell attachment comparable to those seen for unmodified cellulose. Only a minimal level of cationic surface derivitisation (ca 3% degree of substitution) was required for increased cell attachment and no mediating proteins were required. Cell adhesion studies exhibited the same trends as the attachment studies, while the mean cell area and aspect ratio was highest on the cationic surfaces. Overall, we demonstrated the utility of positively charged bacterial cellulose in tissue engineering in the absence of proteins for cell attachment.