HIGH-SPEED PREPARATIVE COUNTERCURRENT CHROMATOGRAPHY WITH A COIL PLANET CENTRIFUGE
HIGH-SPEED PREPARATIVE COUNTERCURRENT CHROMATOGRAPHY WITH A COIL PLANET CENTRIFUGE
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DOI：
10.1016/s0021-9673(00)85688-5
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发表时间：
1982-01-01
期刊：
影响因子：
--
通讯作者：
BOWERS, WG
中科院分区：
文献类型：
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作者：
ITO, Y;SANDLIN, J;BOWERS, WG
A compact table-top model of a flow-through coil planet centrifuge produces an efficient chromatographic separation of solutes on a preparative scale within 2-5 h. The use of a multi-layer coiled column promotes retention of the stationary phase with a high flow-rate of the mobile phase and permits almost universal application of conventional 2-phase solvent sytems. The capability of the counter-current chromatography scheme was demonstrated on separations of various biological samples such as dinitrophenyl amino acids, dipeptides, gramicidins, auxins, purines and pyrimidines.
