Tissue engineering with the aid of inkjet printers
Tissue engineering with the aid of inkjet printers
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DOI：
10.1517/14712598.7.8.1123
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发表时间：
2007-08-01
影响因子：
4.6
通讯作者：
Weiss, Lee E.
中科院分区：
文献类型：
--
作者：
Campbell, Phil G.;Weiss, Lee E.
组织工程有望为组织和器官再生创造革命性的新疗法。这一新兴领域在其各种方法中极为广泛和折衷。然而，正在开发的所有策略都是基于以下类型的组织构建块中的一种或多种的治疗递送：细胞;细胞外基质或支架;以及激素或其他信号分子。到目前为止，大多数工作已经使用这些组分的基本上同质的组合，随后的自组织赋予一定水平的组织功能发生在体外培养期间或移植后。正在研究新兴的“生物打印”方法，以创建最初具有更明确的空间组织的组织工程构建体，其动机是仿生模式可以实现改善的治疗效果的假设。基于喷墨和相关打印技术的生物打印可用于制造持久的仿生图案，这些仿生图案可用于研究组织再生的基础生物学，并可能转化为有效的临床疗法。然而，即使在最原始的水平上重现自然，使得打印的细胞、细胞外基质和激素整合到具有适当功能的分层的、空间组织的三维组织结构中，仍然是一个重大的挑战。
Tissue engineering holds the promise to create revolutionary new therapies for tissue and organ regeneration. This emerging field is extremely broad and eclectic in its various approaches. However, all strategies being developed are based on the therapeutic delivery of one or more of the following types of tissue building-blocks: cells; extracellular matrices or scaffolds; and hormones or other signaling molecules. So far, most work has used essentially homogenous combinations of these components, with subsequent self-organization to impart some level of tissue functionality occurring during in vitro culture or after transplantation. Emerging 'bioprinting' methodologies are being investigated to create tissue engineered constructs initially with more defined spatial organization, motivated by the hypothesis that biomimetic patterns can achieve improved therapeutic outcomes. Bioprinting based on inkjet and related printing technologies can be used to fabricate persistent biomimetic patterns that can be used both to study the underlying biology of tissue regeneration and potentially be translated into effective clinical therapies. However, recapitulating nature at even the most primitive levels such that printed cells, extracellular matrices and hormones become integrated into hierarchical, spatially organized three-dimensional tissue structures with appropriate functionality remains a significant challenge.