Seminal plasma cell-free mitochondrial DNA copy number is associated with human semen quality
Seminal plasma cell-free mitochondrial DNA copy number is associated with human semen quality
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DOI：
10.1016/j.ejogrb.2018.10.048
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发表时间：
2018-12-01
影响因子：
2.6
通讯作者：
Jin, Lei
中科院分区：
文献类型：
--
作者：
Chen, Yuezhou;Liao, Tantan;Jin, Lei
目的：既往研究表明，人类精子中线粒体DNA（mtDNA）拷贝数与精液质量相关。本研究探讨精液游离 mtDNA 拷贝数与人类精液参数之间是否存在关联。研究设计：测量了 205 名男性的精液游离 mtDNA 拷贝数。还测量了精液活性氧（ROS）水平。采用世界卫生组织标准评估精子质量，通过计算机辅助精子分析确定精子活力参数。结果：与正常精子症患者相比，弱精子症和少弱精子症患者游离线粒体DNA拷贝数显着降低，精浆中ROS水平显着升高（均P < 0.05）。精液游离线粒体DNA拷贝数与精子浓度、活力、形态和运动特征呈正相关（r为0.247～0.673，P均<0.05）。精液ROS水平与精子浓度、活力和运动特征呈负相关（r为-0.261至-0.676，P均<0.05）。精液游离线粒体DNA拷贝数与ROS水平呈负相关（r = -0.573，P < 0.05）。结论：结果提示精液游离线粒体DNA拷贝数与精液参数相关，可作为精液质量的新诊断指标。 (C) 2018 Elsevier B.V. 保留所有权利。
Objective: Previous studies have shown that mitochondria] DNA (mtDNA) copy number in human sperm is associated with semen quality. This study examines whether there is association between seminal cell free mtDNA copy number and human semen parameters.Study design: Seminal cell-free mtDNA copy number was measured in 205 men. Semen reactive oxygen species (ROS) level was also measured. Sperm quality was assessed using World Health Organization criteria, while sperm motility parameters were determined by computer-aided sperm analysis.Results: Comparing with normozoospermia, patients with asthenozoospermia and oligoasthenozoo-spermia had a significant decrease in cell-free mtDNA copy number and an increase in ROS level in seminal plasma (P < 0.05 for all). Seminal cell-free mtDNA copy number positively correlated with sperm concentration, motility, morphology and motion characteristics (r for 0.247 to 0.673, P < 0.05 for all). Semen ROS level negatively correlated with sperm concentration, motility, and motion characteristics (r for -0.261 to -0.676, P < 0.05 for all). There is a negative relationship between seminal cell-free mtDNA copy number and ROS level (r = -0.573, P < 0.05).Conclusion: The results suggested that seminal cell-free mtDNA copy number is associated with semen parameters, which may serve as a novel diagnostic marker of semen quality. (C) 2018 Elsevier B.V. All rights reserved.