A bioengineered drug-Eluting scaffold accelerated cutaneous wound healing In diabetic mice
A bioengineered drug-Eluting scaffold accelerated cutaneous wound healing In diabetic mice
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DOI：
10.1016/j.colsurfb.2016.04.056
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发表时间：
2016-09-01
影响因子：
5.8
通讯作者：
Fu，Zhiren
中科院分区：
文献类型：
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作者：
Yin，Hao;Ding，Guoshan;Fu，Zhiren
糖尿病患者的高血压会极大地阻碍伤口愈合过程。在这项研究中，我们研究了F4/80+小鼠巨噬细胞的参与是否可以加速链脲佐菌素诱导的糖尿病小鼠皮肤伤口愈合。为了促进巨噬细胞的参与，我们设计了一种药物洗脱电纺支架，其有效载荷为单核细胞趋化蛋白-1（MCP-1）。MCP-1可以在3天内容易地从支架释放。电纺支架对体外培养的人角质形成细胞无细胞毒性作用。在糖尿病小鼠中产生全层切除皮肤伤口。在用药物洗脱支架治疗的小鼠中，伤口在10天内完全恢复。相比之下，对照组的伤口需要14天才能完全恢复。药物洗脱支架的使用也改善了上皮再形成。此外，我们在第3天用药物洗脱支架治疗的小鼠的伤口床中观察到较大的F4/80+巨噬细胞群体。伤口床中巨噬细胞的显著增加可能有助于加速伤口愈合。我们的研究为糖尿病患者伤口愈合管理的免疫工程解决方案提供了新的思路。
Hyperglycemia in diabetic patients can greatly hinder the wound healing process. In this study we investigated if the engagement of F4/80+murine macrophages could accelerate the cutaneous wound healing in streptozotocin induced diabetic mice. To facilitate the engagement of macrophages, we engineered a drug-eluting electrospun scaffold with a payload of monocyte chemoattractant protein-1 (MCP-1). MCP-1 could be readily released from the scaffold within 3 days. The electrospun scaffold showed no cytotoxic effects on human keratinocytesin vitro. Full-thickness excisional cutaneous wound was created in diabetic mice. The wound fully recovered within 10 days in mice treated with the drug-eluting scaffold. In contrast, the wound took 14 days to fully recover in control groups. The use of drug-eluting scaffold also improved the re-epithelialization. Furthermore, we observed a larger population of F4/80+macrophages in the wound bed of mice treated with drug-eluting scaffolds on day 3. This marked increase of macrophages in the wound bed could have contributed to the accelerated wound healing. Our study shed new light on an immuno-engineering solution for wound healing management in diabetic patients.