MONOCYTE CHEMOTACTIC PROTEIN-1 IS A POTENT ACTIVATOR OF HUMAN BASOPHILS
MONOCYTE CHEMOTACTIC PROTEIN-1 IS A POTENT ACTIVATOR OF HUMAN BASOPHILS
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DOI：
10.1084/jem.175.5.1271
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发表时间：
1992-05-01
影响因子：
15.3
通讯作者：
DAHINDEN, CA
中科院分区：
文献类型：
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作者：
BISCHOFF, SC;KRIEGER, M;DAHINDEN, CA
属于RANTES/SIS家族的细胞因子在许多病理生理过程中被高度诱导，例如自身免疫性疾病、癌症、动脉粥样硬化和慢性炎症。然而，除了它们对单核细胞和特定淋巴细胞类型的趋化活性外，这个最近发现的细胞因子家族在人体系统中的生物活性在很大程度上是未知的。在这里，我们报告说，一个家庭成员，描述为单核细胞趋化蛋白1（MCP-1），强烈激活成熟的人嗜碱性粒细胞在百日咳毒素敏感的方式。MCP-1引起嗜碱性粒细胞和单核细胞胞质游离钙水平升高，但不引起其他血液白细胞类型升高，并在低浓度（ED 50 = 3-10 nM）下触发嗜碱性粒细胞脱粒。因此，MCP-1是能够直接诱导嗜碱性粒细胞释放组胺的细胞因子。此外，MCP-1促进经白细胞介素3（IL-3）、IL-5或粒细胞/巨噬细胞集落刺激因子预处理的嗜碱性粒细胞形成白三烯C4。MCP-1诱导的嗜碱性粒细胞介质释放可能在过敏性炎症和表达MCP-1的其他病理中起重要作用。
Cytokines belonging to the RANTES/SIS family are highly induced in a number of pathophysiological processes such as autoimmune disorders, cancers, atherosclerosis, and chronic inflammation. However, apart from their chemotactic activity on monocytes and particular lymphocyte types, the biological activities in the human system of this recently discovered cytokine family are largely unknown. Here we report that one family member, described as monocyte chemotactic protein 1 (MCP-1), strongly activates mature human basophils in a pertussis toxin-sensitive manner. MCP-1 causes a rise in the cytosolic free calcium level in basophils and monocytes, but not in other blood leukocyte types, and triggers basophil degranulation at low concentrations (ED50 = 3-10 nM). Thus, MCP-1 is a cytokine capable of directly inducing histamine release by basophils. Furthermore, MCP-1 promotes the formation of leukotriene C4 by basophils pretreated with interleukin 3 (IL-3), IL-5, or granulocyte/macrophage colony-stimulating factor. MCP-1-induced basophil mediator release may play an important role in allergic inflammation and other pathologies expressing MCP-1.