Down-regulation of ribosomal protein L7A in human osteosarcoma
Down-regulation of ribosomal protein L7A in human osteosarcoma
复制标题
人骨肉瘤中核糖体蛋白 L7A 的下调
DOI：
10.1007/s00432-008-0538-4
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发表时间：
2009-01
影响因子：
3.6
通讯作者：
Zheng, Shui-er
中科院分区：
文献类型：
--
作者：
Yao, Yang;Zhao, Hui;Dong, Yang;Shen, Zan;Sun, Yuan-jue;Lin, Feng;Tang, Li-na;Zheng, Shui-er
PurposeTo explore the expression of ribosomal protein L7A (RPL7A) in osteosarcoma and its correlation with clinical features.MethodsRibosomal protein L7A mRNA expression was quantified by real-time reverse transcription (RT)-polymerase chain reaction (PCR) in 47 specimens from osteosarcoma, 8 from normal bone tissues and 12 from benign bone lesion tissues. Expression of RPL7A mRNA was also detected in human osteosarcoma cell line MG-63. The relationship between RPL7A mRNA expression and clinicopathological factors was statistically analyzed. The immunoblotting pattern of RPL7A was also analyzed in 20 osteosarcomas, 8 normal bone and 8 benign bone tissues.ResultsRibosomal protein L7A mRNA expression in osteosarcoma samples was significantly down-regulated compared with that in samples from normal bone (P< 0.001) and benign bone lesion tissues (P< 0.001). Low expression of RPL7A mRNA was also found in osteosarcoma cell line MG-63. Low expression of RPL7A mRNA was significantly associated with increased serum level of alkaline phosphatase (ALP,P= 0.008), but was not correlated with other clinicopathological parameters including sex, age, serum lactate dehydrogenase (LDH), tumor location, histological type, histological grading, lung metastasis and overall survival. Interestingly, survival analysis suggested low RPL7A mRNA expression was a significant poor prognostic indicator for overall survival in patients with high grade lesion developed lung metastasis at the time of diagnosis of the primary osteosarcoma (P< 0.05). On western blot, reduced expression of RPL7A protein was observed in osteosarcoma samples (n= 20) compared with normal bone (n= 8) (P< 0.001) and benign bone tissues (n= 8) (P< 0.001).ConclusionUnder-expression of RPL7A may be involved in the carcinogenesis of osteosarcoma. Loss of RPL7A expression may be associated with poor survival of osteosarcoma patients with lung metastasis.
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影响因子：
--
作者：
Miller, CW;Aslo, A;Koeffler, HP
通讯作者：
Koeffler, HP
影响因子：
1.6
作者：
Janov, AJ;Leong, T;Guinan, EC
通讯作者：
Guinan, EC
DOI：
10.1172/jci22320
发表时间：
2005-01
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
A. Minn;Yibin Kang;I. Serganova;G. Gupta;D. Giri;M. Doubrovin;V. Ponomarev;W. Gerald;R. Blasberg-R.-B
通讯作者：
A. Minn;Yibin Kang;I. Serganova;G. Gupta;D. Giri;M. Doubrovin;V. Ponomarev;W. Gerald;R. Blasberg-R.-B
影响因子：
6.4
作者：
Hongxiu Ji;E. Chang;Ken Y. Lin;R. Kurman;D. Pardoll;T. Wu
通讯作者：
Hongxiu Ji;E. Chang;Ken Y. Lin;R. Kurman;D. Pardoll;T. Wu
影响因子：
9.8
作者：
Gazda, Hanna T.;Grabowska, Agnieszka;Sieff, Colin A.
通讯作者：
Sieff, Colin A.