Gga-miR-101-3p Plays a Key Role in Mycoplasma gallisepticum (HS Strain) Infection of Chicken.
Gga-miR-101-3p Plays a Key Role in Mycoplasma gallisepticum (HS Strain) Infection of Chicken.
复制标题
gga-miR-101-3p 在鸡鸡毒支原体（HS 株）感染中发挥关键作用
DOI：
10.3390/ijms161226121
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发表时间：
2015-12-02
影响因子：
5.6
通讯作者：
Peng X
中科院分区：
文献类型：
--
作者：
Chen J;Wang Z;Bi D;Hou Y;Zhao Y;Sun J;Peng X
鸡败支原体（Mycoplasma gallisepticum， MG）是致病性最强的支原体之一，给家禽业造成了巨大的经济损失。近年来，越来越多的证据表明，微核糖核酸（miRNAs）参与了微生物的发病机制。然而，对mirna在鸡MG感染中的潜在作用知之甚少。在本研究中，通过miRNA Solexa测序，我们发现gga-miR-101-3p在mg感染鸡胚胎的肺部中上调。此外，gga-miR-101-3p通过结合EZH2基因的3 ‘非翻译区（3 ’ -UTR）调节zeste同源物2的宿主增强子（EZH2）的表达。过表达gga-miR-101-3p可显著抑制EZH2的表达，从而通过阻断G1-to-S相变抑制鸡胚成纤维细胞（DF-1细胞）的增殖。在mg感染的鸡胚胎和DF-1细胞中也得到了类似的结果，其中gga-miR-101-3p显著上调，而EZH2显著下调。本研究揭示了gga-miR-101-3p通过调控EZH2的表达在MG感染中发挥重要作用，为MG的发病机制提供了新的认识。
Mycoplasma gallisepticum (MG), one of the most pathogenic Mycoplasma, has caused tremendous economic loss in the poultry industry. Recently, increasing evidence has suggested that micro ribonucleic acids (miRNAs) are involved in microbial pathogenesis. However, little is known about potential roles of miRNAs in MG infection of chicken. In the present study, using miRNA Solexa sequencing we have found that gga-miR-101-3p was up-regulated in the lungs of MG-infected chicken embryos. Moreover, gga-miR-101-3p regulated expression of the host enhancer of zeste homolog 2 (EZH2) through binding to the 3’ un-translated region (3’-UTR) of EZH2 gene. Over-expression of gga-miR-101-3p significantly inhibited EZH2 expression and hence inhibited proliferation of chicken embryonic fibroblast (DF-1 cells) by blocking the G1-to-S phase transition. Similar results were obtained in MG-infected chicken embryos and DF-1 cells, where gga-miR-101-3p was significantly up-regulated, while EZH2 was significantly down-regulated. This study reveals that gga-miR-101-3p plays an important role in MG infection through regulation of EZH2 expression and provides a new insight into the mechanisms of MG pathogenesis.