The jasmonate receptor COI1 plays a role in jasmonate-induced lateral root formation and lateral root positioning in Arabidopsis thaliana
The jasmonate receptor COI1 plays a role in jasmonate-induced lateral root formation and lateral root positioning in Arabidopsis thaliana
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DOI：
10.1016/j.jplph.2012.05.002
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发表时间：
2012-09-15
影响因子：
4.3
通讯作者：
Lopez-Bucio, Jose
中科院分区：
文献类型：
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作者：
Raya-Gonzalez, Javier;Pelagio-Flores, Ramon;Lopez-Bucio, Jose
茉莉酸（Jasmonic acid， JA）广泛调控植物的防御和发育反应。COI1最近被发现作为JA受体。在这篇报道中，我们发现低微摩尔浓度的JA抑制了拟南芥野生型（WT）幼苗的初生根（PR）生长，促进了侧根（LR）的形成。结果表明，coi1-1突变体对茉莉酸激活中柱鞘细胞诱导侧根原基（LRP）形成的敏感性降低，侧根定位和侧根出芽发生改变。为了研究JA-生长素对根系结构重塑的重要作用，我们检测了生长素诱导标记DR5:uidA和BA3:uidA在WT和coi -1幼苗中对吲哚-3-乙酸（IAA）和JA的表达，并分析了JA处理下一系列生长素相关突变体的RS结构。我们发现JA对WT和coi1-1幼苗中DR5:uidA和BA3:uidA的表达没有影响。我们的数据还表明，PR对JA的生长抑制可能独立于生长素信号传导，LRP的诱导需要ARF7、ARF19、SLR、T1R1、AFB2、AFB3和AXR1位点。我们得出结论，JA对胚胎后根发育的调控涉及生长素依赖性和独立机制。(c) 2012 Elsevier GmbH版权所有。
Jasmonic acid (JA) regulates a broad range of plant defense and developmental responses. COI1 has been recently found to act as JA receptor. In this report, we show that low micromolar concentrations of JA inhibited primary root (PR) growth and promoted lateral root (LR) formation in Arabidopsis wild-type (WT) seedlings. It was observed that the coi1-1 mutant was less sensitive to JA on pericycle cell activation to induce lateral root primordia (LRP) formation and presented alterations in lateral root positioning and lateral root emergence on bends. To investigate JA-auxin interactions important for remodeling of root system (RS) architecture, we tested the expression of auxin-inducible markers DR5:uidA and BA3:uidA in WT and coi1-1 seedlings in response to indole-3-acetic acid (IAA) and JA and analyzed the RS architecture of a suite of auxin-related mutants under JA treatments. We found that JA did not affect DR5:uidA and BA3:uidA expression in WT and coi1-1 seedlings. Our data also showed that PR growth inhibition in response to JA was likely independent of auxin signaling and that the induction of LRP required ARF7,ARF19, SLR,T1R1, AFB2, AFB3 and AXR1 loci. We conclude that JA regulation of postembryonic root development involves both auxin-dependent and independent mechanisms. (c) 2012 Elsevier GmbH. All rights reserved.