Application and enantioselective residue determination of chiral pesticide penconazole in grape, tea, aquatic vegetables and soil by ultra performance liquid chromatography-tandem mass spectrometry
Application and enantioselective residue determination of chiral pesticide penconazole in grape, tea, aquatic vegetables and soil by ultra performance liquid chromatography-tandem mass spectrometry
复制标题
超高效液相色谱-串联质谱法测定手性农药戊菌唑在葡萄、茶叶、水产蔬菜和土壤中的应用及对映体残留量
DOI：
10.1016/j.ecoenv.2019.01.103
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发表时间：
2019
影响因子：
6.8
通讯作者：
Chen Zongmao
中科院分区：
文献类型：
--
作者：
Zhang Xinzhong;Wang Xinru;Luo Fengjian;Sheng Huishan;Zhou Li;Zhong Qing;Lou Zhengyun;Sun Hezhi;Yang Mei;Cui Xuan;Chen Zongmao
Penconazole is a typical triazole fungicide with wide use on fruits, vegetables, and tea plants to control powdery mildew. In the present study, an efficient graphite carbon black solid phase extraction (GCB-SPE) purification combined with chiral ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed for determination of penconazole enantiomers in different complex matrices, including grape, tea, soil, lotus root, lotus leaf, lotus seed and hulls. The method was then applied to investigate the enantioselective dissipation of penconazole enantiomers in a real field experiment of grape and soil. As a result, a satisfactory separation of penconazole enantiomers on a chiral Lux Cellulose-2 column (150 mm × 2 mm i.d., 3 µm) was obtained with 0.1% formic acid in methanol and 10 mmol L−1ammonium acetate in water (75/25,v/v) as mobile phase at 0.25 mL min−1. The enantiomer (+)-penconazole was firstly eluted, and (-)-penconazole was then eluted. The method showed reliable performances in linearity, recovery and precision, the recoveries of (+)-penconazole and (-)-penconazole in all of six matrices were between 70.5% and 121.0% with the relative standard deviations (RSDs) ranging from 0.8% to 23.6% at the low, medium and high spiked levels. The limits of quantitation (LOQs) of this method were lower than 0.0025 mg kg−1in grape, soil and lotus root, 0.005 mg kg−1in lotus leaf, lotus seed meat and lotus seed shell, and 0.0125 mg kg−1in tea. Results of field trials indicated that (-)-penconazole degraded faster than its (+)-isomer in grape. While only a moderate stereoselectivity was observed in soil, with (-)-penconazole preferential degraded. The proposed method could be used to investigate enantioselective environmental behavior of penconazole enantiomers in complex matrices. And results in this study could provide useful information on realistic risk assessment of penconazole in grape.
