Defective interfering particles in monolayer-propagated Newcastle disease virus.
Defective interfering particles in monolayer-propagated Newcastle disease virus.
复制标题
单层传播的新城疫病毒中有缺陷的干扰颗粒。
DOI：
10.1016/0042-6822(76)90216-6
复制
发表时间：
1976
期刊：
影响因子：
3.7
通讯作者：
E. H. Simon
中科院分区：
文献类型：
--
作者：
J. Roman;E. H. Simon
新城疫病毒(NDV)在鸡胚成纤维细胞(M-NDV)中连续传代会产生缺陷干扰(NDV-DI)颗粒，而在鸡胚蛋中传代(E-NDV)则不会。与这些颗粒和具有感染性的病毒粒子混合感染会导致产量下降99%。干扰不是由于干扰素或阻止传染性病毒粒子的吸收，而是新城疫病毒特有的。以与感染性病毒粒子相同的速度传播干扰沉积物的颗粒。新城疫病毒DI颗粒在单层中积聚，但在鸡蛋中不积聚，这可能是由于M-NDV病毒粒子较大，可能含有更多的RNA，也可能反映了新城疫病毒在鸡蛋和/或单层中复制过程的差异。
Newcastle disease virus (NDV) serially passaged in chick embryo fibroblasts (M-NDV) gives rise to defective interfering (NDV-DI) particles, while NDV passaged in embryonated eggs (E-NDV) does not. Co-infection with these particles and infectious virions results in a 99% reduction in yield. Interference is not due to interferon or to prevention of absorption of infectious virions and is specific for NDV. The particles mediating interference sediment at the same velocity as infectious virions. The accumulation of NDV-DI particles in monolayers but not in eggs may be a consequence of the fact that M-NDV virions are larger and probably contain more RNA, or it may reflect differences in NDV replicative processes in eggs and monolayers, or both.