Improved Pathogenicity of a Beet Black Scorch Virus Variant by Low Temperature and Co-infection with Its Satellite RNA.
Improved Pathogenicity of a Beet Black Scorch Virus Variant by Low Temperature and Co-infection with Its Satellite RNA.
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通过低温和与其卫星RNA的共感染提高了甜菜黑焦病病毒变种的致病性。
DOI：
10.3389/fmicb.2016.01771
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发表时间：
2016
影响因子：
5.2
通讯作者：
Wang XB
中科院分区：
文献类型：
--
作者：
Xu J;Liu D;Zhang Y;Wang Y;Han C;Li D;Yu JL;Wang XB
非编码卫星RNA(sat-RNAs)的混合感染通常会抑制辅助病毒的复制，减轻其疾病症状。然而，我们发现，甜菜黑焦病毒(BBSV)的sat-RNA和低温(18℃)对BBSV在烟草中的系统侵染有相加的作用。Northern印迹杂交显示，与单独使用BBSV相比，在sat-RNA存在的情况下，BBSV衍生的小干扰RNA(SiRNAs)的积累相对减少。小RNA的克隆和测序表明，BBSV-siRNAs占BBSV-siRNAs总序列的50%以上，而Sat-siRNAs的丰度高于BBSV-siRNAs，表明Sat-RNA占据了大多数沉默成分，可能缓解了RNA沉默介导的对BBSV的防御。有趣的是，来自BBSV和Sat-RNA的siRNAs的5‘末端分别由尿苷(U)和腺苷(A)主导。此外，单独感染BBSV和与Sat-RNA一起感染分别诱导MIR168和miR403表达下调，从而导致其靶标ArgAerte1(Ago1)和AGO2的高积累。我们的工作揭示了BBSV和Sat-RNA的siRNAs谱，并为研究辅助病毒和Sat-RNA之间的复杂相互作用提供了额外的线索。
Co-infection of none-coding satellite RNAs (sat-RNAs) usually inhibits replication and attenuates disease symptoms of helper viruses. However, we find that the sat-RNA of Beet black scorch virus (BBSV) and low temperature (18°C) additively enhance the systemic infection of BBSV in Nicotiana benthamiana. Northern blotting hybridization revealed a relatively reduced accumulation of BBSV-derived small interfering RNAs (siRNAs) in presence of sat-RNA as compared to that of BBSV alone. Cloning and sequencing of total small RNAs showed that more than 50% of the total small RNAs sequenced from BBSV-infected plants were BBSV-siRNAs, whereas the abundance of sat-siRNAs were higher than BBSV-siRNAs in the sat-RNA co-infected plants, indicating that the sat-RNA occupies most of the silencing components and possibly relieves the RNA silencing-mediated defense against BBSV. Interestingly, the 5′ termini of siRNAs derived from BBSV and sat-RNA were dominated by Uridines (U) and Adenines (A), respectively. Besides, the infection of BBSV alone and with sat-RNA induce down-regulation of miR168 and miR403, respectively, which leads to high accumulation of their targets, Argonaute 1 (AGO1) and AGO2. Our work reveals the profiles of siRNAs of BBSV and sat-RNA and provides an additional clue to investigate the complicated interaction between the helper virus and sat-RNA.
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