A micro-computed tomography-based method for the measurement of pulmonary compliance in healthy and bleomycin-exposed mice
A micro-computed tomography-based method for the measurement of pulmonary compliance in healthy and bleomycin-exposed mice
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DOI：
10.1080/01902140701364458
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发表时间：
2007-04-01
影响因子：
1.7
通讯作者：
Johnson, G. Allan
中科院分区：
文献类型：
--
作者：
Shofer, Scott;Badea, Cristian;Johnson, G. Allan
微型计算机断层扫描（microCT）正越来越多地用于检查肺损伤的小动物模型。作者开发了一种适用于确定损伤小鼠肺顺应性的microCT技术。正常小鼠的肺体积在吸气末和呼气末进行放射照相测定，并在相隔2周的2个时间点计算肺顺应性，而第二组小鼠给予博来霉素并在给药后3周成像。使用市售呼吸机系统验证顺应性测量。MicroCT肺顺应性测量适用于纵向测量，并与肺顺应性的生理测量相关。
Micro-computed tomography (microCT) is being increasingly used to examine small animal models of pulmonary injury. The authors have developed a microCT technique suitable for the determination of pulmonary compliance in injured mice. Lung volumes in normal mice were radio-graphically determined at end-inspiration and end-expiration and pulmonary compliance was calculated at 2 time points 2 weeks apart, whereas a second group of mice were given bleomycin and imaged 3 weeks following drug administration. Compliance measurements were validated using a commercially available ventilator system. MicroCT pulmonary compliance measurements are suitable for longitudinal measurements, and correlate with physiologic measurements of pulmonary compliance.