Sequence of a cDNA encoding pancreatic preprosomatostatin-22.
Sequence of a cDNA encoding pancreatic preprosomatostatin-22.
复制标题
编码胰腺前促生长抑素-22 的 cDNA 序列。
DOI：
10.1073/pnas.79.17.5152
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发表时间：
1982
影响因子：
11.1
通讯作者：
Dixon,JE
中科院分区：
文献类型：
--
作者：
Magazin,M;Minth,CD;Funckes,CL;Deschenes,R;Tavianini,MA;Dixon,JE
我们报告了生长激素抑制素前体的核苷酸序列，该前体在蛋白水解加工后可能产生22个氨基酸的激素。斑点叉尾（Ictalurus punctatus）的cDNA的核苷酸序列编码生长抑素的前体，其为105个氨基酸（Mr，11，500）。编码生长抑素-22的cDNA由5'非翻译区的36个核苷酸、编码生长抑素-22的前体的315个核苷酸、3'非翻译区的269个核苷酸和可变长度的poly（A）组成。假定的前激素原在氨基末端含有具有“信号”肽性质的疏水氨基酸序列。大约57个氨基酸的连接序列之后是单个Arg-Arg序列，其紧接在激素之前。生长抑素-22在14个氨基酸中的7个氨基酸中与生长抑素-14同源，包括Phe-Trp-Lys序列。mRNA的杂交选择，随后在小麦胚芽无细胞系统中翻译，导致合成了分子量约为10，000的单一多肽，如在Na-DodSO 4/聚丙烯酰胺凝胶上估计的。
We report the nucleotide sequence of a precursor to somatostatin that upon proteolytic processing may give rise to a hormone of 22 amino acids. The nucleotide sequence of a cDNA from the channel catfish (Ictalurus punctatus) encodes a precursor to somatostatin that is 105 amino acids (Mr, 11,500). The cDNA coding for somatostatin-22 consists of 36 nucleotides in the 5' untranslated region, 315 nucleotides that code for the precursor to somatostatin-22, 269 nucleotides at the 3' untranslated region, and a variable length of poly(A). The putative preprohormone contains a sequence of hydrophobic amino acids at the amino terminus that has the properties of a "signal" peptide. A connecting sequence of approximately 57 amino acids is followed by a single Arg-Arg sequence, which immediately precedes the hormone. Somatostatin-22 is homologous to somatostatin-14 in 7 of the 14 amino acids, including the Phe-Trp-Lys sequence. Hybridization selection of mRNA, followed by its translation in a wheat germ cell-free system, resulted in the synthesis of a single polypeptide having a molecular weight of approximately 10,000 as estimated on Na-DodSO4/polyacrylamide gels.