Measurement of trimethylamine and trimethylamine N-oxide independently in urine by fast atom bombardment mass spectrometry
Measurement of trimethylamine and trimethylamine N-oxide independently in urine by fast atom bombardment mass spectrometry
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DOI：
10.1006/abio.1999.4351
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发表时间：
1999-12-15
影响因子：
2.9
通讯作者：
Treacy, EP
中科院分区：
文献类型：
--
作者：
Mamer, OA;Choinière, L;Treacy, EP
本文报道了一种基于快原子轰击质谱（FAB-MS）和稳定同位素稀释技术测定尿中三甲胺（TMA）和N-氧化三甲胺（TMAOx）的方法。从加标N-15标记TMA的尿液中提取TA。将萃取的TMA同位素异构体用三氘代甲基碘季铵化，并在FAB-MS中以六甘醇作为基质进行分析。通过蒸发另一份在FAB探针上加标N-15标记TMAOx的尿液样品来测量TMAOx，并分析TMA。该方法允许准备和简单的区别对照和TMAuria患者，并在监测患者的障碍是有用的。我们给出的例子，它的使用，在确定这些代谢物的正常控制范围，并在评估患者。(C)北京：科学出版社.
We report a method based upon fast atom bombardment mass spectrometry (FAB-MS) and stable isotope dilution techniques for the measurement of urinary trimethylamine (TMA) and trimethylamine N-oxide (TMAOx). TA is extracted from urine that was spiked with N-15-labeled TMA. The extracted TMA isotopomers are quaternized with trideuteromethyl iodide and analyzed in FAB-MS with hexaethylene glycol as matrix. TMAOx is measured by evaporation of another sample of the urine spiked with N-15-labeled TMAOx on the FAB probe and analyzed as for the TMA. The method allows the ready and simple distinguishing of controls and patients with TMAuria, and is useful in monitoring patients with the disorder. We give examples of its use in determining normal control ranges for these metabolites and in evaluating patients. (C) 1999 Academic Press.