Polycyclic aromatic hydrocarbon-degrading Mycobacterium isolates:: their association with plant roots
Polycyclic aromatic hydrocarbon-degrading Mycobacterium isolates:: their association with plant roots
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DOI：
10.1007/s00253-007-0840-0
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发表时间：
2007-06-01
影响因子：
5
通讯作者：
Anderson, A. J.
中科院分区：
文献类型：
--
作者：
Child, R.;Miller, C. D.;Anderson, A. J.
五个环境分枝杆菌菌株，降解多环芳烃（PAHs）与大麦根表面接种种子生长后的幼苗。分枝杆菌细胞检测沿着这些菌株的总根长。这些多环芳烃降解分枝杆菌菌株具有亲水性细胞表面，而一种菌株，MCS，这是疏水性的减少协会沿着根长没有细胞被检测到从根尖。的根尖主管菌株，KMS，是竞争性的，其根协会的存在下，根定殖假单胞菌，恶臭假单胞菌KT 2440。所有的分枝杆菌菌株利用单糖（果糖，葡萄糖）和三糖6-蔗果三糖，存在于大麦根洗涤，用于无菌生长，但它们在体外条件下形成生物膜的潜力不同。矿化的KMS菌株的芘发生时，在大麦根洗的成分进行了修改标记芘，这表明我们矿化可能发生在植物根际含有这样的分枝杆菌菌株。
Five environmental mycobacterium isolates that degrade polycyclic aromatic hydrocarbons (PAHs) were associated with barley root surfaces after growth of the seedlings from inoculated seed. Mycobacterium cells were detected along the total root length for four of these isolates. These PAH-degrading mycobacterium strains had hydrophilic cell surfaces, whereas one strain, MCS, that was hydrophobic had reduced association along the root length with no cells being detected from the root tips. The root-tip-competent strain, KMS, was competitive for its root association in the presence of the root-colonizing pseudomonad, Pseudomonas putida KT2440. All mycobacterium strains utilized simple sugars (fructose, glucose) and the trisaccharide 6-kestose, present in barley root washes, for planktonic growth, but they differed in their potential for biofilm formation under in vitro conditions. Mineralization of pyrene by the KMS strain occurred when the components in the barley root wash were amended with labeled pyrene suggesting to us that mineralization could occur in plant rhizospheres containing such mycobacterium strains.