UTILIZATION OF AMINO-ACIDS BY TRYPANOSOMA-BRUCEI IN CULTURE - L-THREONINE AS A PRECURSOR FOR ACETATE
UTILIZATION OF AMINO-ACIDS BY TRYPANOSOMA-BRUCEI IN CULTURE - L-THREONINE AS A PRECURSOR FOR ACETATE
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DOI：
10.1017/s0031182000046758
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发表时间：
1975-01-01
期刊：
影响因子：
2.4
通讯作者：
LINSTEAD, DJ
中科院分区：
文献类型：
--
作者：
CROSS, GAM;KLEIN, RA;LINSTEAD, DJ
对几种培养基的氨基酸组成进行了分析比较。本文报道了布氏锥虫S42在特定培养基中生长过程中氨基酸和其它代谢产物的利用和排泄。所有添加的L-苏氨酸都被细胞代谢，即使当它以升高的浓度存在时。葡萄糖在整个生长周期中被消耗：谷氨酰胺比谷氨酸消耗得更快，谷氨酸本身的消耗率与脯氨酸大致相同。苏氨酸被裂解形成甘氨酸和乙酸盐，两者都在培养基中积累。丙氨酸和琥珀酸与少量丙酮酸一起排出，但这三种产物占所用葡萄糖的不到一半。没有测量葡萄糖产生的CO2，但苏氨酸产生了少量的CO2。二硫化四乙基秋兰姆阻断了苏氨酸的裂解，是锥虫生长的有效抑制剂。
The amino acid compositions of several culture media have been analysed and compared. The utilization and excretion of amino acids and other metabolites have been followed during growth of Trypanosoma brucei S42 in a defined medium. All of the added L-threonine was metabolized by the cells, even when it was present at elevated concentrations. Glucose was consumed throughout the growth cycle: glutamine was consumed more rapidly than glutamic acid, which was itself used at about the same rate as proline. Threonine was cleaved to form glycine and acetate, both of which accumulated in the medium. Alanine and succinate were excreted together with a small amount of pyruvate, but these three products accounted for less than half of the glucose used. CO2 production from glucose was not measured, but insignificant amounts of CO2 were produced from threonine. Tetraethylthiuram disulphide blocked the cleavage of threonine and was a potent inhibitor of trypanosome growth.