Microglia in culture: What genes do they express?
Microglia in culture: What genes do they express?
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DOI：
10.1159/000080709
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发表时间：
2004-01-01
影响因子：
2.9
通讯作者：
Graeber, MB
中科院分区：
文献类型：
--
作者：
Duke, DC;Moran, LB;Graeber, MB
这项研究中使用的细胞培养模型代表了体外最广泛使用的小胶质细胞范例之一。14天后，从新生大鼠脑中收获的小胶质细胞被认为是“成熟的”。然而，很明显，这是一个有点武断的定义。在这篇文章中，我们提供了这种小胶质细胞的转录组定义。分析了7,000多个已知基因和1,000个表达序列标签簇。“小胶质细胞基因”被定义为在所有被测试的小胶质细胞样本中一致表达的序列。据此，388个基因被鉴定为小胶质细胞基因。在培养物的子集中检测到另外1,440个序列。小胶质细胞持续表达的基因包括已知参与细胞免疫反应、脑组织监测、小胶质细胞迁移和增殖的基因。这里报道的表达谱提供了一个基线，可以用来检测体外培养的小胶质细胞的变化。重要的是，正常小胶质细胞的表达谱将有助于提供目前纯粹可操作的‘小胶质细胞激活’的定义，具有分子生物学相关性。此外，这里报告的数据增加了我们对小胶质细胞生物学的了解，并使我们能够预测小胶质细胞在体内和体外可能发挥的功能。版权所有(C)2004 S.Karger AG，巴塞尔。
The cell culture model utilized in this study represents one of the most widely used paradigms of microglia in vitro. After 14 days, microglia harvested from the neonatal rat brain are considered 'mature'. However, it is clear that this represents a somewhat arbitrary definition. In this paper, we provide a transcriptome definition of such microglial cells. More than 7,000 known genes and 1,000 expressed sequence tag clusters were analysed. 'Microglia genes' were defined as sequences consistently expressed in all microglia samples tested. Accordingly, 388 genes were identified as microglia genes. Another 1,440 sequences were detected in a subset of the cultures. Genes consistently expressed by microglia included genes known to be involved in the cellular immune response, brain tissue surveillance, microglial migration as well as proliferation. The expression profile reported here provides a baseline against which changes of microglia in vitro can be examined. Importantly, expression profiling of normal microglia will help to provide the presently purely operational definition of 'microglial activation' with a molecular biological correlate. Furthermore, the data reported here add to our understanding of microglia biology and allow projections as to what functions microglia may exert in vivo, as well as in vitro. Copyright (C) 2004 S. Karger AG, Basel.