Reduced graphene oxide-coated hydroxyapatite composites stimulate spontaneous osteogenic differentiation of human mesenchymal stem cells
Reduced graphene oxide-coated hydroxyapatite composites stimulate spontaneous osteogenic differentiation of human mesenchymal stem cells
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DOI：
10.1039/c5nr01580d
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发表时间：
2015-01-01
期刊：
影响因子：
6.7
通讯作者：
Han, Dong-Wook
中科院分区：
文献类型：
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作者：
Lee, Jong Ho;Shin, Yong Cheol;Han, Dong-Wook
人骨髓间充质干细胞(HMSCs)作为骨组织工程和再生的细胞来源具有很大的潜力，但其向骨细胞定向分化的控制和诱导仍具有挑战性。基于石墨烯的纳米材料由于其生物相容性和生物活性，被认为是具有吸引力的生物医学应用领域的候选材料，如组织工程中的支架、用于SC分化的基质和可植入设备的部件。尽管石墨烯及其衍生物具有潜在的生物医学应用，但有关它们的成骨活性的信息有限。本研究主要研究了还原氧化石墨烯(RGO)包覆羟基磷灰石(HAP)复合材料对人MSCs成骨分化的影响。HAP和rGO的平均粒径分别为1270+/-476 nm和438+/-180 nm。当rGO包裹在HAP颗粒上时，可以协同促进hMSCs的自发成骨分化，而不会阻碍其增殖。通过测定碱性磷酸酶活性和钙磷矿化作为成骨分化的早期和晚期标志，证实了这一结果。提示rGO包覆的羟基磷灰石复合材料可以有效地作为牙科和骨科的骨填充材料，因为这些基于石墨烯的颗粒材料具有强大的刺激MSCs自发分化的作用，并显示出良好的生物活性和骨诱导潜能。
Human mesenchymal stem cells (hMSCs) have great potential as cell sources for bone tissue engineering and regeneration, but the control and induction of their specific differentiation into bone cells remain challenging. Graphene-based nanomaterials are considered attractive candidates for biomedical applications such as scaffolds in tissue engineering, substrates for SC differentiation and components of implantable devices, due to their biocompatible and bioactive properties. Despite the potential biomedical applications of graphene and its derivatives, only limited information is available regarding their osteogenic activity. This study concentrates upon the effects of reduced graphene oxide (rGO)-coated hydroxyapatite (HAp) composites on osteogenic differentiation of hMSCs. The average particle sizes of HAp and rGO were 1270 +/- 476 nm and 438 +/- 180 nm, respectively. When coated on HAp particulates, rGO synergistically enhanced spontaneous osteogenic differentiation of hMSCs, without hampering their proliferation. This result was confirmed by determining alkaline phosphatase activity and mineralization of calcium and phosphate as early and late stage markers of osteogenic differentiation. It is suggested that rGO-coated HAp composites can be effectively utilized as dental and orthopedic bone fillers since these graphene-based particulate materials have potent effects on stimulating the spontaneous differentiation of MSCs and show superior bioactivity and osteoinductive potential.