Fabrication, nanomechanical characterization, and cytocompatibility of gold-reinforced chitosan bio-nanocomposites.
Fabrication, nanomechanical characterization, and cytocompatibility of gold-reinforced chitosan bio-nanocomposites.
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DOI：
10.1016/j.msec.2014.08.042
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发表时间：
2014-11
期刊：
影响因子：
--
通讯作者：
Yuya PA
中科院分区：
文献类型：
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作者：
Patel NG;Kumar A;Jayawardana VN;Woodworth CD;Yuya PA
壳聚糖是一种天然高分子材料，是工业和生物医学领域中最重要的活性材料之一。通过柠檬酸盐还原法从氯金酸合成金纳米颗粒（~32 nm），并将其掺入壳聚糖基质中。采用溶液浇铸法制备了不同金纳米粒子浓度的生物纳米复合膜。金纳米粒子在整个壳聚糖基质中均匀分布，并用SEM证实。采用SEM、TEM、EDX、SAED、UV-vis、XRD、DLS和Zeta电位等手段对合成产物和制备的纳米复合材料进行了表征。通过纳米压痕技术表征了温度影响下材料的纳米级性质。从准静态纳米压痕，据观察，纳米复合材料的硬度和降低模量显着增加成正比的金纳米粒子的浓度。金纳米颗粒浓度对材料的储存调节和热稳定性也有积极的影响。所获得的薄膜通过其支持体外人类细胞生长的能力被证实具有生物相容性。总之，结果显示随着金纳米颗粒浓度的增加，机械性能增强，并为潜在的生物医学应用提供了更好的理解这种生物相容性材料的结构-性能关系。
Chitosan, a naturally derived polymer represents one of the most technologically important classes of active materials with applications in a variety of industrial and biomedical fields. Gold nanoparticles (~32 nm) were synthesized via a citrate reduction method from chloroauric acid and incorporated in Chitosan matrix. Bio-nanocomposite films with varying concentrations of gold nanoparticles were prepared through solution casting process. Uniform distribution of gold nanoparticles was achieved throughout the chitosan matrix and was confirmed with SEM. Synthesis outcomes and prepared nanocomposites were characterized using SEM, TEM, EDX, SAED, UV–vis, XRD, DLS, and Zeta potential for their physical, morphological and structural properties. Nanoscale properties of materials under the influence of temperature were characterized through nanoindentation techniques. From quasi-static nanoindentation, it was observed that hardness and reduced modulus of the nanocomposites were increased significantly in direct proportion to the gold nanoparticle concentration. Gold nanoparticle concentration also showed positive impact on storagemodulus and thermal stability of the material. The obtained films were confirmed to be biocompatible by their ability to support growth of human cells in vitro. In summary, the results show enhanced mechanical properties with increasing gold nanoparticle concentration, and provide better understanding of the structure–property relationships of such biocompatiblematerials for potential biomedical applications.