Analysis of mainstream tobacco smoke particulate phase using comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry
Analysis of mainstream tobacco smoke particulate phase using comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry
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DOI：
10.1002/jssc.201200812
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发表时间：
2013-03-01
影响因子：
3.1
通讯作者：
Focant, Jean-Francois
中科院分区：
文献类型：
--
作者：
Brokl, Michal;Bishop, Louise;Focant, Jean-Francois
综合二维气相色谱与飞行时间质谱联用用于表征主流烟草烟雾颗粒的颗粒相。在标准化条件下，在旋转吸烟机上吸食五支 3R4F 研究卷烟，在剑桥过滤垫上收集总颗粒物，并使用基于甲醇的液体萃取和动态顶空 (DHS) 方法进行提取。使用自动峰值查找和质谱解卷积结合脚本编写和库命中的手动修订来评估库搜索结果。修订后的峰表包含 DHS 样品的近 1800 种单独化合物和溶剂提取样品的 900 多种化合物。这些提取方法被证明是互补的，仅产生 11% 的重复分析物，而它们的组合产生了近 2500 种单独化合物的列表。
Comprehensive 2D GC coupled to time-of-flight mass spectrometry was applied for the characterization of the particulate phase of mainstream tobacco smoke particulate. Five 3R4F research cigarettes were smoked on a rotary smoking machine under standardized conditions, total particular matter was collected on Cambridge filter pads and extracted using methanol-based liquid extraction and dynamic headspace (DHS) approaches. Automated peak finding and mass spectral deconvolution combined with scripting and manual revision of library hits were used to evaluate the library search results. The revised peak table contained nearly 1800 individual compounds for the DHS sample and over 900 for the solvent extracted sample. These methods of extraction were shown to be complementary, leading to only 11% of repeated analytes, and their combination gave rise to a list of almost 2500 individual compounds.