Carboxyl-fentanyl detection using optical fibre grating-based sensors functionalised with molecularly imprinted nanoparticles
Carboxyl-fentanyl detection using optical fibre grating-based sensors functionalised with molecularly imprinted nanoparticles
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DOI：
10.1016/j.bios.2021.113002
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发表时间：
2021-01-21
影响因子：
12.6
通讯作者：
Korposh, Serhiy
中科院分区：
文献类型：
--
作者：
Liu, LiangLiang;Grillo, Fabiana;Korposh, Serhiy
丁酰芬太尼是一种新的设计药物，据报道，其使用和相关死亡人数不断增加。尚未建立这种新型合成阿片类药物的常规毒理学分析。这项工作报告了一种光纤传感器，测量羧基芬太尼，这是丁酰芬太尼的主要代谢产物在血液中，提供了一个很有前途的工具，用于检测丁酰芬太尼中毒。采用相位匹配的长周期光纤光栅（LPG）传感器阵列与最先进的分子印迹技术相结合。纳米分子印迹聚合物（nanoMIPs）是通过固相方法合成的，并涂覆在LPG阵列的表面上。LPG阵列由两部分组成：检测LPG和参考LPG。前者在测量之前用nanoMIPs官能化，而后者用于考虑检测LPG的温度响应。所开发的传感器在羧基芬太尼的浓度从0增加到1000 ng/mL时表现出逐渐的响应，最小检测浓度为50 ng/mL，对应于1.20 +/- 0.2 nm的波长偏移。应用Langmuir吸附等温线拟合分析数据，其显示结合常数为2.03 μ M-1。所开发的传感器在检测其他药物和潜在的干扰物，包括吗啡，可卡因，葡萄糖和白蛋白之间的羧基芬太尼显示出高选择性。它与芬太尼有一定的交叉反应，因为芬太尼与羧基芬太尼具有相同的结合位点，因此可以用于芬太尼的检测。
Butyrylfentanyl is a new designer drug reported with growing use and related deaths. Routine toxicological analyses of this novel synthetic opioid drug have not been established yet. This work reports a fibre optic sensor that measures carboxyl-fentanyl which is the major metabolite of butyrylfentanyl presented in blood, providing a promising tool for detecting butyrylfentanyl intoxication. A long period fibre grating (LPG) sensor array operating at phase-matching condition is deployed in combination with a state-of-the-art molecular imprinting technique. Nano-sized molecularly imprinted polymers (nanoMIPs) are synthesised via a solid-phase approach and coated on the surface of an LPG array. An LPG array consists of two parts: a detection and a reference LPG. The former is functionalised with nanoMIPs prior to the measurements, whilst the latter is used to take into account the temperature response of the detection LPG. The developed sensor exhibits a gradual response over increasing concentrations of carboxyl-fentanyl from 0 to 1000 ng/mL with a minimal detected concentration of 50 ng/mL, that corresponds to a wavelength shift of 1.20 +/- 0.2 nm. The Langmuir adsorption isotherm is applied to fit the analytical data which reveal a binding constant of 2.03 mu M-1. The developed sensor shows high selectivity in detecting carboxyl-fentanyl among other drugs and potential interferents including morphine, cocaine, glucose and albumin. It shows a certain degree of cross-response to fentanyl which shares the same binding sites as carboxyl-fentanyl and therefore can be potentially used to detect fentanyl.